BUFFER, pH 7.2 (Merck), tab. for 1 l

STD SLASBUFF7P2

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W0105900201 - Buffers (unassignable ), supplem. reagents, etc
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BUFFER, pH 7.2 (Merck), tab. for 1 l

Definition

Pre-measured reagent to prepare the buffer solution (pH 7.2 ± 0,2 = neutral) used to dilute Giemsa intended for staining blood smears and blood samples.

Specifications

  • Tablet for 1 litre
  • Solid, white tablets
  • Component:
    • Na2HPO4 x 2H2O - 0.63 g/L
    • KH2PO4 - 0.31 g/L

Packaging & Labelling

Per 100 tablets

Instructions for use

A buffer solution is not necessary if the water has already a stable pH 7.2.

Giemsa stains are generally diluted 1:10 in the buffer solution.

Once they are reconstituted, the Giemsa solutions can be kept only for some hours. They need to be prepared extemporaneously, in small quantities (maximum 5 ml per slide).

Always check the stability of the reconstituted solutions with pH strips.

Please consult the “Updated laboratory procedures, 2022” available online via the Laboratory working Group sharepoint page: Laboratory Procedures and Resources.

https://msfintl.sharepoint.com/sites/msfintlcommunities/LabWG/SitePages/Laboratory-Manual-page.aspx

For offline access, contact your laboratory advisor.

Storage

  • Expiry date indicated on the bottle
  • In case of need the expiry date can be passed as long as the buffer solution is clear and has a correct pH value (to check with pH strip).
  • Protected from humidity
  • Store between 5 and 30°C
  • Buffer solution can be used for at least 4 weeks when kept in a tightly closed glass bottle

MSF requirements

Essential buffer solution to perform a quality Giemsa staining.

Pre-measured reagent (no scale is required).

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