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BUFFER, pH 7.2 (Merck), tab. for 1 l

https://unicat.msf.org/cat/product/18748
STD SLASBUFF7P2

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Former Code(s): -X
CE marking: declaration that the product meets EU standards for health, safety, and environmental protection. The CE marking indicates that the product may be sold freely in any part of the European Economic Area, regardless of its country of origin.
W0105900201 - Buffers (unassignable ), supplem. reagents, etc
European Medical Device Nomenclature (EMDN) is the nomenclature of use by manufacturers when registering their medical devices in the EUDAMED database. EMDN is characterised by its alphanumeric structure that is established in a seven-level hierarchical tree.
CT30
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The product is part of at least one Kit. A kit is a collection of products (medical and/or logistic) that are needed for a certain intervention in emergency. The choice and quantity of the articles reflects the MSF protocols for this specific situation. The use of Kits allows to start an intervention without a detailed evaluation.
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BUFFER, pH 7.2 (Merck), tab. for 1 l

Definition

Pre-measured reagent to prepare the buffer solution (pH 7.2 ± 0,2 = neutral) used to dilute Giemsa intended for staining blood smears and blood samples.

Specifications

  • Tablet for 1 litre
  • Solid, white tablets
  • Component:
    • Na2HPO4 x 2H2O - 0.63 g/L
    • KH2PO4 - 0.31 g/L

Packaging & Labelling

Per 100 tablets

Instructions for use

A buffer solution is not necessary if the water has already a stable pH 7.2.

Giemsa stains are generally diluted 1:10 in the buffer solution.

Once they are reconstituted, the Giemsa solutions can be kept only for some hours. They need to be prepared extemporaneously, in small quantities (maximum 5 ml per slide).

Always check the stability of the reconstituted solutions with pH strips.

Please consult the “Updated laboratory procedures, 2022” available online via the Laboratory working Group sharepoint page: Laboratory Procedures and Resources.

https://msfintl.sharepoint.com/sites/msfintlcommunities/LabWG/SitePages/Laboratory-Manual-page.aspx

For offline access, contact your laboratory advisor.

Storage

  • Expiry date indicated on the bottle
  • In case of need the expiry date can be passed as long as the buffer solution is clear and has a correct pH value (to check with pH strip).
  • Protected from humidity
  • Store between 5 and 30°C
  • Buffer solution can be used for at least 4 weeks when kept in a tightly closed glass bottle

MSF requirements

Essential buffer solution to perform a quality Giemsa staining.

Pre-measured reagent (no scale is required).

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