BUFFER, pH 7.2 (Merck), tab. for 1 l
Valid Article
BUFFER, pH 7.2 (Merck), tab. for 1 l
Definition
Pre-measured reagent to prepare the buffer solution (pH 7.2 ± 0,2 = neutral) used to dilute Giemsa intended for staining blood smears and blood samples.
Specifications
- Tablet for 1 litre
- Solid, white tablets
- Component:
- Na2HPO4 x 2H2O - 0.63 g/L
- KH2PO4 - 0.31 g/L
Packaging & Labelling
Per 100 tablets
Instructions for use
A buffer solution is not necessary if the water has already a stable pH 7.2.
Giemsa stains are generally diluted 1:10 in the buffer solution.
Once they are reconstituted, the Giemsa solutions can be kept only for some hours. They need to be prepared extemporaneously, in small quantities (maximum 5 ml per slide).
Always check the stability of the reconstituted solutions with pH strips.
Please consult the “Updated laboratory procedures, 2022” available online via the Laboratory working Group sharepoint page: Laboratory Procedures and Resources.
https://msfintl.sharepoint.com/sites/msfintlcommunities/LabWG/SitePages/Laboratory-Manual-page.aspx
For offline access, contact your laboratory advisor.
Storage
- Expiry date indicated on the bottle
- In case of need the expiry date can be passed as long as the buffer solution is clear and has a correct pH value (to check with pH strip).
- Protected from humidity
- Store between 5 and 30°C
- Buffer solution can be used for at least 4 weeks when kept in a tightly closed glass bottle
MSF requirements
Essential buffer solution to perform a quality Giemsa staining.
Pre-measured reagent (no scale is required).