ILAB - Laboratory checklists

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CL Laboratory safety and infection control

23 Product(s)
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Definition

List of essential articles to guarantee safety and infection control in a laboratory.

Instructions for use

A safety cabinet is only recommended in certain situations. Contact your laboratory referent at headquarters for further information.

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CL Blood collection venipuncture

19 Product(s)
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Used Techniques

Venipuncture in vacuum sterile tubes.

There is an associated module with all material needed for safety blood sampling, in context of haemorragic fever.

Instructions for use

Closed system to prevent any contact with blood.

It is recommended to wear gloves for any handling of blood samples.

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CL Haemoglobin

11 Product(s)
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Used Techniques

Haemoglobin photometer: HemoCue Hb 301 (tropicalized model)

Instructions for use

Use only microcuvettes which are designed for the photometer. They are different for the Hb 301 and the Hb 201+ models.

Do not forget equipment for blood collection (capillary or venous)

It is recommended to wear gloves for any handling of blood samples.

Please consult the “Updated laboratory procedures, 2022” available online via the Laboratory working Group sharepoint page: Laboratory Procedures and Resources.

https://msfintl.sharepoint.com/sites/msfintlcommunities/LabWG/SitePages/Laboratory-Manual-page.aspx

For offline access, contact your laboratory advisor.

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CL haematocrit

6 Product(s)
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Used Techniques

Determination of haematocrit with electrical centrifuge.

Instructions for use

Capillary or venous sample in EDTA tube. Do not forget equipment for blood collection.

Used for concentration techniques in trypanosomiasis programmes.

The haematocrit capillary tubes should be sealed before centrifuging with sealing paste.

It is recommended to wear gloves for any handling of blood samples.

Please consult the “Updated laboratory procedures, 2022” available online via the Laboratory working Group sharepoint page: Laboratory Procedures and Resources.

https://msfintl.sharepoint.com/sites/msfintlcommunities/LabWG/SitePages/Laboratory-Manual-page.aspx

For offline access, contact your laboratory advisor.

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CL Cryptococcus, CSF, Indian ink

9 Product(s)
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Used Techniques

Examination of CSF:

  • Using India ink for cryptococcus detection.
  • Rapid immunochromatographic test (possible also on serum, plasma or whole blood) with possible qualitative and semi-quantitative titration procedures‌ for cryptococcus.
  • Pandy test (detection of increased levels of globulin in CSF).

Instructions for use

It is strongly recommended to use an electrical centrifuge.

Do not forget the material and equipment for sample collection.

It is recommended to wear gloves for any handling of blood samples.

Please consult the “Updated laboratory procedures, 2022” available online via the Laboratory working Group sharepoint page: Laboratory Procedures and Resources.

https://msfintl.sharepoint.com/sites/msfintlcommunities/LabWG/SitePages/Laboratory-Manual-page.aspx

For offline access, contact your laboratory advisor.

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CL cryptococcus, rapid test

11 Product(s)
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Used Techniques

Examination of CSF:

  • Using India ink for cryptococcus detection.
  • Rapid immunochromatographic test (possible also on serum, plasma or whole blood) with possible qualitative and semi-quantitative titration procedures‌ for cryptococcus.
  • Pandy test (detection of increased levels of globulin in CSF).

Instructions for use

It is strongly recommended to use an electrical centrifuge.

Do not forget the material and equipment for sample collection.

It is recommended to wear gloves for any handling of blood samples.

Please consult the “Updated laboratory procedures, 2022” available online via the Laboratory working Group sharepoint page: Laboratory Procedures and Resources.

https://msfintl.sharepoint.com/sites/msfintlcommunities/LabWG/SitePages/Laboratory-Manual-page.aspx

For offline access, contact your laboratory advisor.

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CL Test trypanosomiasis (CATT)

16 Product(s)
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Used Techniques

Rapid serological test (CATT test) used for the screening of Trypanosoma gambiense (sleeping sickness).

Instructions for use

The CATT is the only screening test for trypanosomiasis. The diagnosis of trypanosomiasis must be completed with other tests:

  • liquid of ganglionary puncture
  • Woo test: looking for trypanosoma in a blood sample
  • lumbar puncture (double centrifuge method) to determine the stage of the disease

This list includes the equipment needed for the CATT test on whole blood and the CATT test dilution. Do not forget equipment for sample collection.

It is recommended to wear gloves for any handling of blood samples.

Please consult the “Updated laboratory procedures, 2022” available online via the Laboratory working Group sharepoint page: Laboratory Procedures and Resources.

https://msfintl.sharepoint.com/sites/msfintlcommunities/LabWG/SitePages/Laboratory-Manual-page.aspx

For offline access, contact your laboratory advisor.

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CL Kala-Azar test (DAT)

26 Product(s)
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Used Techniques

Rapid serological test (DAT) used for the detection of visceral leishmaniasis (Kala-Azar) in serum or whole blood.

Instructions for use

Do not forget equipment for blood sample collection (capillary or venous).

It is recommended to wear gloves for any handling of blood samples.

Please consult the “Updated laboratory procedures, 2022” available online via the Laboratory working Group sharepoint page: Laboratory Procedures and Resources.

https://msfintl.sharepoint.com/sites/msfintlcommunities/LabWG/SitePages/Laboratory-Manual-page.aspx

For offline access, contact your laboratory advisor.

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CL stain malaria, filariasis, leishmaniasis

24 Product(s)
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Used Techniques

Thin / thick blood film stained with Giemsa for the detection of malaria, borreliasis, leishmaniasis, filariasis.

Instructions for use

The Giemsa solution should be extemporaneously prepared. The racks for staining and drying slides can be locally manufactured.

Malaria, filariasis and borreliasis are generally done with a capillary blood sample (we can also use venous blood). Do not forget equipment for blood sample collection.

It is recommended to wear gloves for any handling of blood samples.

Leishmaniasis needs an lymph node or spleen puncture, what requires a particular training which is organized only in some specific programmes.

Visceral leishmaniasis (Kala-Azar) requires in addition another diagnostic method with a serological test.

Please consult the “Updated laboratory procedures, 2022” available online via the Laboratory working Group sharepoint page: Laboratory Procedures and Resources.

https://msfintl.sharepoint.com/sites/msfintlcommunities/LabWG/SitePages/Laboratory-Manual-page.aspx

For offline access, contact your laboratory advisor.

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CL skin microfilariasis (onchocerciasis)

13 Product(s)
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Used Techniques

Detection of onchocerciasis on bloodless skin biopsy.

Instructions for use

For the sodium chloride solution, use a normal saline infusion instead of the powder to be diluted (need of a spatula, measuring cylinder and scale).

It is recommended to wear gloves for any handling of samples.

Please consult the “Updated laboratory procedures, 2022” available online via the Laboratory working Group sharepoint page: Laboratory Procedures and Resources.

https://msfintl.sharepoint.com/sites/msfintlcommunities/LabWG/SitePages/Laboratory-Manual-page.aspx

For offline access, contact your laboratory advisor.

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CL drepanocytosis, Emmel test

14 Product(s)
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Used Techniques

Emmel test (= sickle cell test) on whole blood/ capillary blood with sodium disulfite (metabisulfite) or saline.

Instructions for use

The technique with sodium disulfite is recommended as it is more sensitive than saline.

A scale is needed to prepare the reagent (2% solution: 0.5 g for 25 ml distilled water).

The metabisulphite solution is unstable and can be kept maximum 8 hours.

Alternative: test with paraffine.

It is recommended to wear gloves during the test.

See also the checklist for blood collection/venipuncture for the necessary material for sampling.

Please consult the “Updated laboratory procedures, 2022” available online via the Laboratory working Group sharepoint page: Laboratory Procedures and Resources.

https://msfintl.sharepoint.com/sites/msfintlcommunities/LabWG/SitePages/Laboratory-Manual-page.aspx

For offline access, contact your laboratory advisor.

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CL CSF, proteins, Pandy test

11 Product(s)
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Used Techniques

Examination of CSF:

  • Using India ink for cryptococcus detection.
  • Rapid immunochromatographic test (possible also on serum, plasma or whole blood) with possible qualitative and semi-quantitative titration procedures‌ for cryptococcus.
  • Pandy test (detection of increased levels of globulin in CSF).

Instructions for use

It is strongly recommended to use an electrical centrifuge.

Do not forget the material and equipment for sample collection.

It is recommended to wear gloves for any handling of blood samples.

Please consult the “Updated laboratory procedures, 2022” available online via the Laboratory working Group sharepoint page: Laboratory Procedures and Resources.

https://msfintl.sharepoint.com/sites/msfintlcommunities/LabWG/SitePages/Laboratory-Manual-page.aspx

For offline access, contact your laboratory advisor.

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CL TB, microscopy sputum examination Ziehl Neelsen

40 Product(s)
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Used Techniques

Smears stained with Ziehl-Neelsen for microscopic detection of mycobacteria.

Instructions for use

Wearing a mask is mandatory during the preparation of the smear. It is also mandatory to wear gloves for any handling of samples.

CAUTION: never pipet by mouth!

The rack for slides can be manufactured locally.

Other possible staining: fluorescence system with auramine staining. Contact your laboratory referent for more information on performing auramine staining.

NB: The TB culture checklists are in the TB culture catalogue.

Please consult the “Updated laboratory procedures, 2022” available online via the Laboratory working Group sharepoint page: Laboratory Procedures and Resources.

https://msfintl.sharepoint.com/sites/msfintlcommunities/LabWG/SitePages/Laboratory-Manual-page.aspx

For offline access, contact your laboratory advisor.

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CL TB, microscopy sputum exam. Auramine

24 Product(s)
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Used Techniques

Staining technique using the fluorescent auramine reagent for microscopic detection of mycobacteria.

Instructions for use

Wearing a mask is mandatory during the preparation of the smear. It is also mandatory to wear gloves for any handling of samples.

CAUTION: never pipet by mouth!

The rack for slides can be manufactured locally.

Other possible staining: staining with Ziehl Neelsen. Contact your laboratory referent for more information.

NB: The TB culture checklists are in the TB culture catalogue.

Please consult the “Updated laboratory procedures, 2022” available online via the Laboratory working Group sharepoint page: Laboratory Procedures and Resources.

https://msfintl.sharepoint.com/sites/msfintlcommunities/LabWG/SitePages/Laboratory-Manual-page.aspx

For offline access, contact your laboratory advisor.

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CL parasitology of stools

18 Product(s)
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Used Techniques

Direct stools examination.

Instructions for use

For lack of stools sample containers, use urine sample containers [ELAECONT6U-].

Please consult the “Updated laboratory procedures, 2022” available online via the Laboratory working Group sharepoint page: Laboratory Procedures and Resources.

https://msfintl.sharepoint.com/sites/msfintlcommunities/LabWG/SitePages/Laboratory-Manual-page.aspx

For offline access, contact your laboratory advisor.

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CL examination of urine, biochemistry

5 Product(s)
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Used Techniques

  • Biochemistry : urine strips: glucose, proteins, pH, density, ketones, blood, nitrites, leucocytes.
  • Direct examination of urine sediment for cell count and detection of parasites (trichomonas, bilharzia).

Instructions for use

Two different strips are available; with 2 and 8 parameters (see checklist). Select the strips according to the parameters to be determined.

Please consult the “Updated laboratory procedures, 2022” available online via the Laboratory working Group sharepoint page: Laboratory Procedures and Resources.

https://msfintl.sharepoint.com/sites/msfintlcommunities/LabWG/SitePages/Laboratory-Manual-page.aspx

For offline access, contact your laboratory advisor.

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CL exam. of urine, cell count + sediment

12 Product(s)
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Used Techniques

  • Biochemistry : urine strips: glucose, proteins, pH, density, ketones, blood, nitrites, leucocytes.
  • Direct examination of urine sediment for cell count and detection of parasites (trichomonas, bilharzia).

Instructions for use

Two different strips are available; with 2 and 8 parameters (see checklist). Select the strips according to the parameters to be determined.

Please consult the “Updated laboratory procedures, 2022” available online via the Laboratory working Group sharepoint page: Laboratory Procedures and Resources.

https://msfintl.sharepoint.com/sites/msfintlcommunities/LabWG/SitePages/Laboratory-Manual-page.aspx

For offline access, contact your laboratory advisor.

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CL clinical chemistry analyzer i-STAT

25 Product(s)
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Definition

The i-STAT is a handheld device for the automated, quantitative determination of clinical chemistry parameters using cartridges for single use. Cartridges with a different configuration panels for the analysis of blood gases, electrolytes, coagulation, hematology, glucose and creatinine are available.

Instructions for use

Quality control cartridges should be used at least once daily.

Do not forget equipment for blood sample collection (capillary, venous, arterial).

It is recommended to wear gloves for any handling of blood samples.

Please consult the “Updated laboratory procedures, 2022” available online via the Laboratory working Group sharepoint page: Laboratory Procedures and Resources.

https://msfintl.sharepoint.com/sites/msfintlcommunities/LabWG/SitePages/Laboratory-Manual-page.aspx

For offline access, contact your laboratory advisor.

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CL CD4 analyzer PIMA (Abbott)

12 Product(s)
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Precatalog Text (English)

The PIMA CD4 analyzer has been discontinued by the manufacturer in May 2022.

The consumables and spare parts remain available. Contact your laboratory advisor for alternative options.

Definition

PIMA analyser is a benchtop and portable fixed-volume cytometer for the enumeration of CD4+ T cells with PIMA CD4 test cartridge on capillary or venous blood.

Instructions for use

Do not forget the equipment for blood sampling with EDTA tube (see checklist blood collection).

If the test is performed on capillary blood, the ordering of the sample collection kit is mandatory, because it contains specific lancets for PIMA CD4 use.

Please consult the “Updated laboratory procedures, 2022” available online via the Laboratory working Group sharepoint page: Laboratory Procedures and Resources.

https://msfintl.sharepoint.com/sites/msfintlcommunities/LabWG/SitePages/Laboratory-Manual-page.aspx

For offline access, contact your laboratory advisor.

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CL CD4 FACSCount® (Becton Dickinson)

25 Product(s)
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Precatalog Text (English)

BD FacsCount CD4 analyzer have been discontinued by the manufacturer. Contact your laboratory advisor for alternative options.

The reagents and consumables for the FacsCount remain available up to end of 2023.

Definition

BD FACSCount is a flow cytometer for the enumeration of CD4+ (and CD3+, CD8+) T cells and the measurement of the percentage of CD4+ T cells among all lymphocytes in blood.

Instructions for use

Do not forget the equipment for blood sampling with EDTA tube (see checklist blood collection)

Please consult the “Updated laboratory procedures, 2022” available online via the Laboratory working Group sharepoint page: Laboratory Procedures and Resources.

https://msfintl.sharepoint.com/sites/msfintlcommunities/LabWG/SitePages/Laboratory-Manual-page.aspx

For offline access, contact your laboratory advisor.

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CL ELISA reader for TSH monitoring

19 Product(s)
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Definition

Enzyme-linked immunosorbent assay (ELISA) method to qualitatively and/or quantitatively determine the presence of a protein through absorbance using a microplate reader.
This reader can be used to measure absorbance with multiple ELISA plate formats.

In MSF, the ELISA test is currently used to monitor thyroid stimulating hormone (TSH) in MDR TB patients, in high case loads of some serological tests and for operational research.

Instructions for use

The ELISA washer is reserved for programmes with high ELISA analysis throughput (many plates) and good infrastructure.

Do not forget equipment for venous blood sample collection.

Please consult the “Updated laboratory procedures, 2022” available online via the Laboratory working Group sharepoint page: Laboratory Procedures and Resources.

https://msfintl.sharepoint.com/sites/msfintlcommunities/LabWG/SitePages/Laboratory-Manual-page.aspx

For offline access, contact your laboratory advisor.

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CL haematology analyzer SYSMEX XP300/XQ320

14 Product(s)
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Precatalog Text (English)

The haematology analyzer XQ-320 replaces the XP-300 starting from 2023. The XQ-320 uses the same reagents and solutions as the XP-300 but spare parts are different for both models.

Definition

Haematology analysers are used for full blood counts: total white blood cell count, red blood cell count and platelets. In addition, the analyser measures also haemoglobin, haematocrit, percentage of lymphocytes and granulated white blood cells.

Instructions for use

Do not forget the equipment for blood sampling with EDTA tubes (see checklist blood collection).

Please consult the “Updated laboratory procedures, 2022” available online via the Laboratory working Group sharepoint page: Laboratory Procedures and Resources.

https://msfintl.sharepoint.com/sites/msfintlcommunities/LabWG/SitePages/Laboratory-Manual-page.aspx

For offline access, contact your laboratory advisor.

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CL Molecular biology GeneXpert GX-IV MTB

9 Product(s)
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Precatalog Text (English)

The MTB/RIF cartridge will be discontinued by the manufacturer in December 2023 and replaced by the MTB/RIF Ultra cartridge.

Definition

The GeneXpert is an automated molecular cartridge based, closed system to perform nucleic acid amplification testing (NAAT).

The test Xpert MTB/RIF and MTB/RIF Ultra cartridge is used to detect Mycobacterium tuberculosis and rifampicine resistance. The MTB/RIF Ultra has a lower limit of detection than MTB/RIF, reduces false rifampicin resistance and better differentiate silent from resistant-conferring mutations.

The test Xpert MTB/XDR is used to detect extensively drug resistant Mycobacterium tuberculosis, with resistance detection to isoniazid, ethionamide, fluoroquinolone, second line injectable drug.

Instructions for use

The GeneXpert® MTB test does not eliminate the need for conventional microscopy, culture and DST, which are required to monitor treatment progress and to detect resistance of Mycobacterium tuberculosis to drugs.

The operating temperature for GeneXpert device is 15-30°C.

Please consult the “Updated laboratory procedures, 2022” available online via the Laboratory working Group sharepoint page: Laboratory Procedures and Resources.

https://msfintl.sharepoint.com/sites/msfintlcommunities/LabWG/SitePages/Laboratory-Manual-page.aspx

For offline access, contact your laboratory advisor.

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CL Clin. chemistry spectrophotometer Humalyzer 2000 (Human)

44 Product(s)
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Precatalog Text (English)

The Humalyzer 2000 has been stopped by the manufacturer. Contact your laboratory advisor for alternative options.

The support for Humalyzer 2000 will be discontinued in November 2023, and spare parts for this instrument will only be available as long as stock lasts.

Definition

A biochemistry analyser (commonly called "spectrophotometer") is used to carry out different clinical and haematology analyses such as liver function test (ALAT, ASAT), renal function test (creatinine, bilirubine) etc.

Instructions for use

Specific kits allow to carry out each analysis. It is compulsory to use the controls.

The dosage kits and control solutions are thermosensitive items: they must be transported by cold chain and kept refrigerated between 2 and 8°C.

Do not forget equipment for venous blood sample collection.

Please consult the “Updated laboratory procedures, 2022” available online via the Laboratory working Group sharepoint page: Laboratory Procedures and Resources.

https://msfintl.sharepoint.com/sites/msfintlcommunities/LabWG/SitePages/Laboratory-Manual-page.aspx

For offline access, contact your laboratory advisor.

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CL sample digestion/decontamination

24 Product(s)
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Instructions for use

Several items in this checklist are NST items. Please contact your referents to ensure all needed material is available.

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CL culture Lowenstein-Jensen medium

48 Product(s)
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Instructions for use

For additional equipment (e.g. lamp) and NST consummables (e.g. potassium phosphate), please contact your referents to ensure all needed material is available.

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CL culture Thin Layer Agar technique

59 Product(s)
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Instructions for use

Several items in this checklist are NST items. Please contact your referents to ensure all needed material is available.

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CL mycobacterial culture MGIT manual

34 Product(s)
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Instructions for use

Several items in this checklist are NST items. Please contact your referents to ensure all needed material is available.

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CL drug susceptibility test LJ

25 Product(s)
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Instructions for use

Several items in this checklist are NST items. Please contact your referents to ensure all needed material is available.

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CL drug susceptibility test MGIT manual

33 Product(s)
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Instructions for use

Several items in this checklist are NST items. Please contact your referents to ensure all needed material is available.

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CL drug susceptibility test MGIT 960

55 Product(s)
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Instructions for use

Several items in this checklist are NST items. Please contact your referents to ensure all needed material is available.

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CL identification M. tuberculosis

22 Product(s)
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Instructions for use

One item in this checklist is NST. Please contact your referents to ensure all needed material is available.

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CL HbA1C analyser

11 Product(s)
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Definition

The HemoCue HbA1c is an automated portable device for the quantitative measurement of glycated haemoglobin (HbA1c) in whole blood. The measurement is based on the boronate affinity method and results are measured photometrically. The test is used to evaluate the long-term control of blood glucose concentration, primarily in persons with diabetes mellitus.

Instructions for use

  • Daily check: 1/day ==> cartridge is reusable for 24 months
  • Monthly check: 1 / month ==> cartridge is single use
  • 3 control solutions: 1 / week

Do not forget the equipment for blood collection (capillary or venous)

Please consult the “Updated laboratory procedures, 2022” available online via the Laboratory working Group sharepoint page: Laboratory Procedures and Resources.

https://msfintl.sharepoint.com/sites/msfintlcommunities/LabWG/SitePages/Laboratory-Manual-page.aspx

For offline access, contact your laboratory advisor.

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CL GeneXpert EID

12 Product(s)
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Definition

The GeneXpert is an automated molecular cartridge based, closed system to perform nucleic acid amplification testing (NAAT). One of the two cartridges currently being used, is the Xpert HIV-1 Qual XC EID for the qualitative in vitro detection of HIV-1 total nucleic acids using whole blood and dried blood spot. In MSF programs this test shall be used for early infant diagnosis of HIV-1.

The Xpert® HIV-1 XC Qual assay is not intended to be used as screening test of blood donors for HIV-1.

Instructions for use

  • The operating temperature for GeneXpert device is 15-30°C.
  • Standard safety precautions for handling human specimens should be followed when carrying out this test.
  • The Eppendorf Thermomixer and Eppendorf Smartblock in the checklist are only needed for the DBS application.
  • Do not forget equipment for blood sample collection (venous or capillary).

Please consult the “Updated laboratory procedures, 2022” available online via the Laboratory working Group sharepoint page: Laboratory Procedures and Resources.

https://msfintl.sharepoint.com/sites/msfintlcommunities/LabWG/SitePages/Laboratory-Manual-page.aspx

For offline access, contact your laboratory advisor.

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CL molecular biology HAIN LPA equipment

13 Product(s)
.

Definition

Line probe assays (LPA) are a family of DNA strip-based tests that allow users to determine the drug resistance profile of an MTB strain by interpreting a pattern of bands that represent lines of immobilized probes that are bound (or hybridized) to MTB amplicons (DNA amplification products). LPA probes are designed to target the most common mutations associated with resistance to first- and second-line anti-TB agents and specific MTBC wild-type (WT) DNA sequences.

This checklist details a set of materials needed for the drug susceptibility testing of M. tuberculosis and the differentiation between M. tuberculosis and 27 nontuberculous Mycobacteria;

  • GenoType MTBDRplus VER 2.0: M. tuberculosis complex and its resistances to rifampicin and isoniazid from specimens or cultures
  • GenoType MTBDRsl VER 2.0: M. tuberculosis complex and its resistances to fluoroquinolones and injectable antibiotics (kanamycin, amikacin, capreomycin, and viomycin) from specimens or cultures
  • GenoType Mycobacterium CM VER 2.0: Detection of M. tuberculosis complex and 27 clinically relevant NTM from cultures

Instructions for use

Mycobacterial DNA is extracted from sputum specimens or cultivated material, specifically amplified via PCR and detected on a membrane strip using reverse hybridization and an enzymatic colour reaction. Valid results are documented by internal controls, Conjugate and Amplification Control.

Molecular testing requires specific laboratory set up, equipment and trained personnel. Refer to your lab advisor.

HAIN LPA does not eliminate the need for conventional microscopy, culture and DST, which are required to monitor treatment success and to assess resistance to other drugs.

Standard safety precautions for handling human specimens should be followed when carrying out this test. Always wear suitable protective clothing and gloves. Handling of potentially infectious specimens must be carried out in a class II safety cabinet.

No Product(s) in your list!

CL TB sputum specimen storage and shipment

11 Product(s)
.

Definition

List of essential articles for specimens storage and shipment.

Instructions for use

Specimens shipment should always follow triple packaging requirements to ensure biosafety.

Specimens must be stored refrigerated.

External shipment of specimens to reference laboratories must follow IATA regulations.

Remarks:

Articles for storage and transport of isolates are not included in this list.

No Product(s) in your list!

CL molecular biology HAIN LPA small equipment

11 Product(s)
.

Definition

Line probe assays (LPA) are a family of DNA strip-based tests that allow users to determine the drug resistance profile of an MTB strain by interpreting a pattern of bands that represent lines of immobilized probes that are bound (or hybridized) to MTB amplicons (DNA amplification products). LPA probes are designed to target the most common mutations associated with resistance to first- and second-line anti-TB agents and specific MTBC wild-type (WT) DNA sequences.

This checklist details a set of materials needed for the drug susceptibility testing of M. tuberculosis and the differentiation between M. tuberculosis and 27 nontuberculous Mycobacteria;

  • GenoType MTBDRplus VER 2.0: M. tuberculosis complex and its resistances to rifampicin and isoniazid from specimens or cultures
  • GenoType MTBDRsl VER 2.0: M. tuberculosis complex and its resistances to fluoroquinolones and injectable antibiotics (kanamycin, amikacin, capreomycin, and viomycin) from specimens or cultures
  • GenoType Mycobacterium CM VER 2.0: Detection of M. tuberculosis complex and 27 clinically relevant NTM from cultures

Instructions for use

Mycobacterial DNA is extracted from sputum specimens or cultivated material, specifically amplified via PCR and detected on a membrane strip using reverse hybridization and an enzymatic colour reaction. Valid results are documented by internal controls, Conjugate and Amplification Control.

Molecular testing requires specific laboratory set up, equipment and trained personnel. Refer to your lab advisor.

HAIN LPA does not eliminate the need for conventional microscopy, culture and DST, which are required to monitor treatment success and to assess resistance to other drugs.

Standard safety precautions for handling human specimens should be followed when carrying out this test. Always wear suitable protective clothing and gloves. Handling of potentially infectious specimens must be carried out in a class II safety cabinet.

No Product(s) in your list!

CL molecular biology HAIN LPA reagents

6 Product(s)
.

Definition

Line probe assays (LPA) are a family of DNA strip-based tests that allow users to determine the drug resistance profile of an MTB strain by interpreting a pattern of bands that represent lines of immobilized probes that are bound (or hybridized) to MTB amplicons (DNA amplification products). LPA probes are designed to target the most common mutations associated with resistance to first- and second-line anti-TB agents and specific MTBC wild-type (WT) DNA sequences.

This checklist details a set of materials needed for the drug susceptibility testing of M. tuberculosis and the differentiation between M. tuberculosis and 27 nontuberculous Mycobacteria;

  • GenoType MTBDRplus VER 2.0: M. tuberculosis complex and its resistances to rifampicin and isoniazid from specimens or cultures
  • GenoType MTBDRsl VER 2.0: M. tuberculosis complex and its resistances to fluoroquinolones and injectable antibiotics (kanamycin, amikacin, capreomycin, and viomycin) from specimens or cultures
  • GenoType Mycobacterium CM VER 2.0: Detection of M. tuberculosis complex and 27 clinically relevant NTM from cultures

Instructions for use

Mycobacterial DNA is extracted from sputum specimens or cultivated material, specifically amplified via PCR and detected on a membrane strip using reverse hybridization and an enzymatic colour reaction. Valid results are documented by internal controls, Conjugate and Amplification Control.

Molecular testing requires specific laboratory set up, equipment and trained personnel. Refer to your lab advisor.

HAIN LPA does not eliminate the need for conventional microscopy, culture and DST, which are required to monitor treatment success and to assess resistance to other drugs.

Standard safety precautions for handling human specimens should be followed when carrying out this test. Always wear suitable protective clothing and gloves. Handling of potentially infectious specimens must be carried out in a class II safety cabinet.

No Product(s) in your list!

CL molecular biology HAIN LPA consumables

10 Product(s)
.

Definition

Line probe assays (LPA) are a family of DNA strip-based tests that allow users to determine the drug resistance profile of an MTB strain by interpreting a pattern of bands that represent lines of immobilized probes that are bound (or hybridized) to MTB amplicons (DNA amplification products). LPA probes are designed to target the most common mutations associated with resistance to first- and second-line anti-TB agents and specific MTBC wild-type (WT) DNA sequences.

This checklist details a set of materials needed for the drug susceptibility testing of M. tuberculosis and the differentiation between M. tuberculosis and 27 nontuberculous Mycobacteria;

  • GenoType MTBDRplus VER 2.0: M. tuberculosis complex and its resistances to rifampicin and isoniazid from specimens or cultures
  • GenoType MTBDRsl VER 2.0: M. tuberculosis complex and its resistances to fluoroquinolones and injectable antibiotics (kanamycin, amikacin, capreomycin, and viomycin) from specimens or cultures
  • GenoType Mycobacterium CM VER 2.0: Detection of M. tuberculosis complex and 27 clinically relevant NTM from cultures

Instructions for use

Mycobacterial DNA is extracted from sputum specimens or cultivated material, specifically amplified via PCR and detected on a membrane strip using reverse hybridization and an enzymatic colour reaction. Valid results are documented by internal controls, Conjugate and Amplification Control.

Molecular testing requires specific laboratory set up, equipment and trained personnel. Refer to your lab advisor.

HAIN LPA does not eliminate the need for conventional microscopy, culture and DST, which are required to monitor treatment success and to assess resistance to other drugs.

Standard safety precautions for handling human specimens should be followed when carrying out this test. Always wear suitable protective clothing and gloves. Handling of potentially infectious specimens must be carried out in a class II safety cabinet.

No Product(s) in your list!

CL TB culture laboratory safety and infection control

29 Product(s)
.

Definition

List of essential articles to guarantee safety and infection control in a tuberculosis culture laboratory.

No Product(s) in your list!
PRODUCT INFO Quantity
1
1
1
100
100
100
1
1
1
1
1
1
1
1
1
1
1
1
1
50
1
1
100
100
100
100
50
50
50

Antimicrobial sensitivity panel - Strepto A, B, C, G

5 Product(s)
.

Definition

To evaluate the in vitro susceptibility to antimicrobial agents of Streptococcus, group A, B, C and G by an agar diffusion method.

No Product(s) in your list!

Antimicrobial sensitivity testing Consumables

4 Product(s)
.

Definition

Mueller Hinton medium is used for standardised testing of antimicrobial sensitivity testing (antibiogram with agar diffusion method).

If it is complemented with horse blood/sheep blood, it is used for AST for fastidious microorganisms.

No Product(s) in your list!

Antimicrobial sensitivity testing Material/Equipment

7 Product(s)
.

Definition

Mueller Hinton medium is used for standardised testing of antimicrobial sensitivity testing (antibiogram with agar diffusion method).

If it is complemented with horse blood/sheep blood, it is used for AST for fastidious microorganisms.

No Product(s) in your list!

Reagents for API STAPH strip

5 Product(s)
.

Definition

API reagents to be used with specifically with API strips.

Caution, in some cases, reagents are already included in kit (API NH).

Sterile normal saline solution 0.9% (DEXOSODC9D5) can be used instead of the proposed API NaCl 0.85% (SBIDAPISNACL2).

No Product(s) in your list!

Reagents for API 20E strip

6 Product(s)
.

Definition

API reagents to be used with specifically with API strips.

Caution, in some cases, reagents are already included in kit (API NH).

Sterile normal saline solution 0.9% (DEXOSODC9D5) can be used instead of the proposed API NaCl 0.85% (SBIDAPISNACL2).

No Product(s) in your list!

Antimicrobial sensitivity panel - Strepto viridans

6 Product(s)
.

Definition

To evaluate the in vitro susceptibility to antimicrobial agents of Streptococcus viridans by an agar diffusion method.

No Product(s) in your list!

Antimicrobial sensitivity panel - Enteroccocus

6 Product(s)
.

Definition

To evaluate the in vitro susceptibility to antimicrobial agents of Enterococcus sp by an agar diffusion method.

No Product(s) in your list!

Antimicrobial sensitivity panel - Strepto pneumoniae

9 Product(s)
.

Definition

To evaluate the in vitro susceptibility to antimicrobial agents of Streptococcus pneumoniae by an agar diffusion method.

Instructions for use

MIC Cefotaxime should be tested only for neonates

No Product(s) in your list!

Antimicrobial sensitivity panel - Listeria sp

4 Product(s)
.

Definition

To evaluate the in vitro susceptibility to antimicrobial agents of Listeria sp by an agar diffusion method.

No Product(s) in your list!

Antimicrobial sensitivity panel - Enterobacteria

18 Product(s)
.

Definition

To evaluate the in vitro susceptibility to antimicrobial agents of Enterobacteriacea by an agar diffusion method.

Instructions for use

In case of production of carbapenemase (resistance to Ertapenem), Tigecycline MIC can be tested only after validation of the infectious disease referent and the microbiology referent.

No Product(s) in your list!

Antimicrobial sensitivity panel - Non fermenting bact

13 Product(s)
.

Definition

To evaluate the in vitro susceptibility to antimicrobial agents of non fermenting bacteria by an agar diffusion method.

Instructions for use

Tigecycline MIC and Colistin ComAST can be tested only in case of resistance to imipenem and only after validation of the infectious disease referent and the microbiology referent.

No Product(s) in your list!

Antimicrobial sensitivity panel - Haemophilus sp

7 Product(s)
.

Definition

To evaluate the in vitro susceptibility to antimicrobial agents of Haemophilus sp by an agar diffusion method.

No Product(s) in your list!

Antimicrobial sensitivity panel - ESBL

4 Product(s)
.

Definition

For the phenotypic detection of ESBL (extended-spectrum beta-lactamase) in case of resistance to third generation cephalosporines.

No Product(s) in your list!

Culture media preparation - Agar - Equipment

7 Product(s)
.

Definition

Mueller Hinton medium is used for standardised testing of antimicrobial sensitivity testing (antibiogram with agar diffusion method).

If it is complemented with horse blood/sheep blood, it is used for AST for fastidious microorganisms.

No Product(s) in your list!

Culture media preparation - Tube - Equipment

7 Product(s)
.

Definition

Material, equipement, consumables and reagents needed for the preparation of Kligler Iron agar culture media in glass tubes.

No Product(s) in your list!

Preparation broth media - Equipment

4 Product(s)
.

Definition

Equipement, material, consumables and reagents for the storage of bacterial strains in laboratory.

No Product(s) in your list!

Culture media preparation (Agar)-Consumables/small material

6 Product(s)
.

Definition

Material, equipement and consumables needed for the preparation of culture media in Petri dishes.

No Product(s) in your list!

Culture media preparation (Tube)-Consumables/small material

7 Product(s)
.

Definition

Material, equipement, consumables and reagents needed for the preparation of Kligler Iron agar culture media in glass tubes.

No Product(s) in your list!

Storage bacterial strains (broth)-Consumable/small material

11 Product(s)
.

AST Culture Media preparation - Consumables/small material

9 Product(s)
.

Definition

Mueller Hinton medium is used for standardised testing of antimicrobial sensitivity testing (antibiogram with agar diffusion method).

If it is complemented with horse blood/sheep blood, it is used for AST for fastidious microorganisms.

No Product(s) in your list!

Chromagar culture media - Reagents

1 Product(s)
.

Definition

Reagents needed for the preparation of the culture media in Petri dishes.

No Product(s) in your list!

CNA culture media - Reagents

1 Product(s)
.

Definition

Reagents needed for the preparation of the culture media in Petri dishes.

No Product(s) in your list!
PRODUCT INFO Quantity
1

Chocolate culture media - Reagents

3 Product(s)
.

Definition

Reagents needed for the preparation of the culture media in Petri dishes.

No Product(s) in your list!

MacConkey culture media - Reagents

1 Product(s)
.

Definition

Reagents needed for the preparation of the culture media in Petri dishes.

No Product(s) in your list!

Columbia Blood culture media - Reagents

1 Product(s)
.

Definition

Reagents needed for the preparation of the culture media in Petri dishes.

No Product(s) in your list!
PRODUCT INFO Quantity
1

Mueller-Hinton AST culture media - Reagents

1 Product(s)
.

Definition

Mueller Hinton medium is used for standardised testing of antimicrobial sensitivity testing (antibiogram with agar diffusion method).

If it is complemented with horse blood/sheep blood, it is used for AST for fastidious microorganisms.

No Product(s) in your list!

Kligler Iron culture media - Reagents

1 Product(s)
.

Definition

Material, equipement, consumables and reagents needed for the preparation of Kligler Iron agar culture media in glass tubes.

No Product(s) in your list!

Brain-Heart Broth - Reagents

2 Product(s)
.

Definition

Equipement, material, consumables and reagents for the storage of bacterial strains in laboratory.

No Product(s) in your list!

Reagents for API 20NE strip

4 Product(s)
.

Definition

API reagents to be used with specifically with API strips.

Caution, in some cases, reagents are already included in kit (API NH).

Sterile normal saline solution 0.9% (DEXOSODC9D5) can be used instead of the proposed API NaCl 0.85% (SBIDAPISNACL2).

No Product(s) in your list!

Sterilisation - autoclave ALL AMERICAN 39L

13 Product(s)
.

CL Cold chain Bacteriology

8 Product(s)
.

Definition

All needed material for the cold chain in the microbiology laboratory, to store consumables and bacterial strains.

For each laboratory, as a minimum and depending of the level of activity, there must be:

  • one vertical fridge
  • one fridge Verstfrost
  • one freezer

Check with the microbiology referent what equipement is needed and which are references for these equipments

No Product(s) in your list!

Haemoculture sampling and manipulation

23 Product(s)
.

Definition

Material needed for haemoculture collection.

No Product(s) in your list!
PRODUCT INFO Quantity
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1

Reagents for API NH strip

3 Product(s)
.

Definition

API reagents to be used with specifically with API strips.

Caution, in some cases, reagents are already included in kit (API NH).

Sterile normal saline solution 0.9% (DEXOSODC9D5) can be used instead of the proposed API NaCl 0.85% (SBIDAPISNACL2).

No Product(s) in your list!

Bones and Tissues sampling and inoculation

33 Product(s)
.

Definition

Material used for the collection and inoculation of bones and tissues for bacterial culture.

All material should be single use or sterile.

No Product(s) in your list!
PRODUCT INFO Quantity
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1

Sheep blood collection and manipulation

12 Product(s)
.

Culture media - commun items

5 Product(s)
.

Definition

List of all items needed in the laboratory to perform bacteriology analyses.

Instructions for use

Depending to what is used in clinic, choose Imipenem or Meropenem.

No Product(s) in your list!

AM susceptibility testing - commun items

41 Product(s)
.

Definition

List of all items needed in the laboratory to perform bacteriology analyses.

Instructions for use

Depending to what is used in clinic, choose Imipenem or Meropenem.

No Product(s) in your list!
PRODUCT INFO Quantity
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1

AM susceptibility - items to validate

2 Product(s)
.

Definition

List of all items needed in the laboratory to perform bacteriology analyses.

Instructions for use

Depending to what is used in clinic, choose Imipenem or Meropenem.

No Product(s) in your list!

Bacteriology laboratory - commun material

53 Product(s)
.

Definition

List of all items needed in the laboratory to perform bacteriology analyses.

Instructions for use

Depending to what is used in clinic, choose Imipenem or Meropenem.

No Product(s) in your list!
PRODUCT INFO Quantity
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1

Bacteriology laboratory - commun equipment + acccessories

12 Product(s)
.

Bacteriology laboratory - Quality control strains

16 Product(s)
.

Definition

List of all items needed in the laboratory to perform bacteriology analyses.

Instructions for use

Depending to what is used in clinic, choose Imipenem or Meropenem.

No Product(s) in your list!

Specific material/reagents for haemoculture

21 Product(s)
.

Definition

List of specific items for each kind of culture realised in a bacteriology laboratory

No Product(s) in your list!
PRODUCT INFO Quantity
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1

Material/reagent specific for urine culture

4 Product(s)
.

Definition

List of specific items for each kind of culture realised in a bacteriology laboratory

No Product(s) in your list!

Specific material/reagents for bones and tissues culture

20 Product(s)
.

Definition

List of specific items for each kind of culture realised in a bacteriology laboratory

No Product(s) in your list!

Antimicrobial sensitivity panel - Neisseria

4 Product(s)
.

Definition

To evaluate the in vitro susceptibility to antimicrobial agents of Neisseria by an agar diffusion method.

No Product(s) in your list!

CL WBC differential analyzer

5 Product(s)
.

Definition

Device for near patient quantitative and qualitative determination of white blood cells (WBC) in capillary or venous whole blood.

The machine gives the number of white blood cells and the 5-parameter formula (polynuclear: neutrophils, eosinophils, basophils, lymphocytes and monocytes).

Instructions for use

3 control solutions: 1 / week

Do not forget the equipment for blood sampling (venous ou capillary blood).

Please consult the “Updated laboratory procedures, 2022” available online via the Laboratory working Group sharepoint page: Laboratory Procedures and Resources.

https://msfintl.sharepoint.com/sites/msfintlcommunities/LabWG/SitePages/Laboratory-Manual-page.aspx

For offline access, contact your laboratory advisor.

No Product(s) in your list!

CL CD4 Analyzer FACSPresto (Becton Dickinson)

11 Product(s)
.

Precatalog Text (English)

The FacsPresto will be discontinued by the manufacturer by December 2023, last orders to be placed by September 2023. Contact your laboratory advisor for alternative options.

The last order for the cartridges is March 2024, and the last shipment July 2024.

The service and support will continue up until June 2026.

Definition

The BD FACSPresto™ CD4 analyser provides absolute and percentage results of CD4 T lymphocytes and total haemoglobin (Hb) concentration in whole blood samples.

Instructions for use

Do not forget the equipment for blood sampling with EDTA tube (see checklist blood collection)

Please consult the “Updated laboratory procedures, 2022” available online via the Laboratory working Group sharepoint page: Laboratory Procedures and Resources.

https://msfintl.sharepoint.com/sites/msfintlcommunities/LabWG/SitePages/Laboratory-Manual-page.aspx

For offline access, contact your laboratory advisor.

No Product(s) in your list!

CL Molecular biology RT-PCR GeneXpert GX-IV MTB/RIF Ultra

11 Product(s)
.

Definition

The GeneXpert is an automated molecular cartridge based, closed system to perform nucleic acid amplification testing (NAAT). The test Xpert MTB/RIF Ultra cartridge is used to detect Mycobacterium tuberculosis and rifampicine resistance.

Instructions for use

The GeneXpert® MTB/RIF Ultra test does not eliminate the need for conventional microscopy, culture and DST, which are required to monitor treatment progress and to detect resistance of Mycobacterium tuberculosis to other drugs than rifampicin.

The operating temperature for GeneXpert device is 15-30°C.

Please consult the “Updated laboratory procedures, 2022” available online via the Laboratory working Group sharepoint page: Laboratory Procedures and Resources.

https://msfintl.sharepoint.com/sites/msfintlcommunities/LabWG/SitePages/Laboratory-Manual-page.aspx

For offline access, contact your laboratory advisor.

No Product(s) in your list!

CL GeneXpert HIV VL

12 Product(s)
.

Definition

The GeneXpert is an automated molecular cartridge based, closed system to perform nucleic acid amplification testing (NAAT). The Xpert HIV-1 VL is used for the in vitro quantification of HIV-1 total nucleic acids using whole blood.

The Xpert® HIV-1 VL assay is not intended to be used as screening test of blood donors for HIV-1.

Instructions for use

  • The operating temperature for GeneXpert device is 15-30°C.
  • Standard safety precautions for handling human specimens should be followed when carrying out this test.
  • Do not forget equipment for venous blood sample collection.
  • Wear gloves when manipulating samples
No Product(s) in your list!

CL GeneXpert HCV VL

9 Product(s)
.

Definition

The GeneXpert is an automated molecular cartridge based, closed system to perform nucleic acid amplification testing (NAAT). The Xpert HCV VL is used for the in vitro quantification of HCV total nucleic acids using whole blood.

The Xpert® HCV VL assay is not intended to be used as screening test of blood donors for HCV.

Instructions for use

  • The operating temperature for GeneXpert device is 15-30°C.
  • Standard safety precautions for handling human specimens should be followed when carrying out this test.
  • Do not forget equipment for venous blood sample collection.

Please consult the “Updated laboratory procedures, 2022” available online via the Laboratory working Group sharepoint page: Laboratory Procedures and Resources.

https://msfintl.sharepoint.com/sites/msfintlcommunities/LabWG/SitePages/Laboratory-Manual-page.aspx

For offline access, contact your laboratory advisor.

No Product(s) in your list!

CL Clinical chemistry NYCOCARD CRP

9 Product(s)
.

Definition

The Nycocard Reader II is an handheld instrument for quantitative determination of CRP.

Instructions for use

Do not forget equipment for blood collection (venous or capillary)

Please consult the “Updated laboratory procedures, 2022” available online via the Laboratory working Group sharepoint page: Laboratory Procedures and Resources.

https://msfintl.sharepoint.com/sites/msfintlcommunities/LabWG/SitePages/Laboratory-Manual-page.aspx

For offline access, contact your laboratory advisor.

No Product(s) in your list!

CL Capillary blood collection

13 Product(s)
.

Used Techniques

Capillary blood collection with lancets, with (or without) microtainer tubes.

Instructions for use

It is recommended to wear gloves for any handling of blood samples.

Different lancets available:

  • STSSLANCSAM2: SAFETY LANCET, medium flow, needle 21G x 1.8mm, green, s.u.
    • Use: Rapid tests and handheld analysers in infants, children and adults.
    • Blood volume: 10-50µl.
    • Puncture site: heel (up to 6 months ) finger
  • STSSLANCSH3: SAFETY LANCET, high flow, blade 1.5 X 1.2mm, pink, s.u.
    • Use: For DBS for HIV EID and VL in infants, children and adults.
    • Blood volume: 50-250µl.
    • Puncture site: heel (up to 6 months) finger
  • STSSLANCSHL1 - SAFETY LANCET, low flow, needle 28Gx1.5mm, light blue, s.u.
    • Use: rapid tests and handheld analysers in infants and children.
    • Blood volume: 10-20µl.
    • Puncture site: heel (up to 6 months) finger
No Product(s) in your list!

CL Glucometer Nova StatStrip

8 Product(s)
.

Definition

MSF has different types of small portable electronic chemistry analyzers; two glucometers, a creatinometer, and a lactatometer. These handheld analyzers are validated for near patient testing by medical staff and in specific settings glucometers can be used for self-testing to monitor glycemia at home. All analyzers are battery operated, easy and quick to use, and require a small volume of blood.

Instructions for use

  • Always ensure that the strips are compatible with the analyzer
  • Choose a handheld analyzer adapted to the needs (near patient use or home use) and reporting results with the desired unit' (e.g. glucometer are available in mmol/l and in mg/dl)
  • Do not forget material for blood sample collection

Please consult the “Updated laboratory procedures, 2022” available online via the Laboratory working Group sharepoint page: Laboratory Procedures and Resources.

https://msfintl.sharepoint.com/sites/msfintlcommunities/LabWG/SitePages/Laboratory-Manual-page.aspx

For offline access, contact your laboratory advisor.

No Product(s) in your list!

CL Glucometer AccuCheck Instant

4 Product(s)
.

Definition

MSF has different types of small portable electronic chemistry analyzers; two glucometers, a creatinometer, and a lactatometer. These handheld analyzers are validated for near patient testing by medical staff and in specific settings glucometers can be used for self-testing to monitor glycemia at home. All analyzers are battery operated, easy and quick to use, and require a small volume of blood.

Instructions for use

  • Always ensure that the strips are compatible with the analyzer
  • Choose a handheld analyzer adapted to the needs (near patient use or home use) and reporting results with the desired unit' (e.g. glucometer are available in mmol/l and in mg/dl)
  • Do not forget material for blood sample collection

Please consult the “Updated laboratory procedures, 2022” available online via the Laboratory working Group sharepoint page: Laboratory Procedures and Resources.

https://msfintl.sharepoint.com/sites/msfintlcommunities/LabWG/SitePages/Laboratory-Manual-page.aspx

For offline access, contact your laboratory advisor.

No Product(s) in your list!

CL Creatininometer Nova StatSensor

6 Product(s)
.

Definition

MSF has different types of small portable electronic chemistry analyzers; two glucometers, a creatinometer, and a lactatometer. These handheld analyzers are validated for near patient testing by medical staff and in specific settings glucometers can be used for self-testing to monitor glycemia at home. All analyzers are battery operated, easy and quick to use, and require a small volume of blood.

Instructions for use

  • Always ensure that the strips are compatible with the analyzer
  • Choose a handheld analyzer adapted to the needs (near patient use or home use) and reporting results with the desired unit' (e.g. glucometer are available in mmol/l and in mg/dl)
  • Do not forget material for blood sample collection

Please consult the “Updated laboratory procedures, 2022” available online via the Laboratory working Group sharepoint page: Laboratory Procedures and Resources.

https://msfintl.sharepoint.com/sites/msfintlcommunities/LabWG/SitePages/Laboratory-Manual-page.aspx

For offline access, contact your laboratory advisor.

No Product(s) in your list!

CL Lactatometer Accutrend Plus

4 Product(s)
.

Definition

MSF has different types of small portable electronic chemistry analyzers; two glucometers, a creatinometer, and a lactatometer. These handheld analyzers are validated for near patient testing by medical staff and in specific settings glucometers can be used for self-testing to monitor glycemia at home. All analyzers are battery operated, easy and quick to use, and require a small volume of blood.

Instructions for use

  • Always ensure that the strips are compatible with the analyzer
  • Choose a handheld analyzer adapted to the needs (near patient use or home use) and reporting results with the desired unit' (e.g. glucometer are available in mmol/l and in mg/dl)
  • Do not forget material for blood sample collection

Please consult the “Updated laboratory procedures, 2022” available online via the Laboratory working Group sharepoint page: Laboratory Procedures and Resources.

https://msfintl.sharepoint.com/sites/msfintlcommunities/LabWG/SitePages/Laboratory-Manual-page.aspx

For offline access, contact your laboratory advisor.

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CL Piccolo

9 Product(s)
.

Definition

The Piccolo Xpress analyzer is a portable clinical chemistry instrument.

Instructions for use

Do not forget equipment for venous blood collection and centrifuge.

Please consult the “Updated laboratory procedures, 2022” available online via the Laboratory working Group sharepoint page: Laboratory Procedures and Resources.

https://msfintl.sharepoint.com/sites/msfintlcommunities/LabWG/SitePages/Laboratory-Manual-page.aspx

For offline access, contact your laboratory advisor.

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CL Spotchem EL (Arkray)

6 Product(s)
.

Definition

The Spotchem EL SE-1520 is an electrolyte measurement system for measurement of sodium (Na+) , potassium (K+) and chloride (Cl-).

Instructions for use

Do not forget equipment for venous blood collection and centrifuge or collection of urine sample.

Please consult the “Updated laboratory procedures, 2022” available online via the Laboratory working Group sharepoint page: Laboratory Procedures and Resources.

https://msfintl.sharepoint.com/sites/msfintlcommunities/LabWG/SitePages/Laboratory-Manual-page.aspx

For offline access, contact your laboratory advisor.

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CL Blood Grouping - 1st choice

7 Product(s)
.

Precatalog Text (English)

The 5 reagents for blood grouping (anti-AB, anti-A, anti-B, anti Rh D, Rhesus negative control) must all be used and come from the same manufacturer

Definition

ABO reagents contain immunoglobulins used for the blood group typing by determining the presence or absence of antigens on the red cells.

Instructions for use

Do not forget equipment for blood collection (venous or capillary)

It is recommended to wear gloves for any handling of blood sample

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CL Blood grouping - 2nd choice

7 Product(s)
.

Precatalog Text (English)

The 5 reagents for blood grouping (anti-AB, anti-A, anti-B, anti Rh D, Rhesus negative control) must all be used and come from the same manufacturer

Definition

ABO reagents contain immunoglobulins used for the blood group typing by determining the presence or absence of antigens on the red cells.

Instructions for use

Do not forget equipment for blood collection (venous or capillary)

It is recommended to wear gloves for any handling of blood sample

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CL Pastorex - meningitis A, B, C, Y/W135

10 Product(s)
.

Used Techniques

Rapid test for the detection of bacteria responsible for meningitis in CSF (cerebro spinal fluid).

Detects the meningococcus A, B, C, Y/W135, the streptococcus B and pneumoniae, as well as the Haemophilus influenzae B.

Instructions for use

  • Do not forget material for lumbar puncture

Please consult the “Updated laboratory procedures, 2022” available online via the Laboratory working Group sharepoint page: Laboratory Procedures and Resources.

https://msfintl.sharepoint.com/sites/msfintlcommunities/LabWG/SitePages/Laboratory-Manual-page.aspx

For offline access, contact your laboratory advisor.

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CL CATT Test on whole blood

4 Product(s)
.

Definition

Human african trypanosomiasis [HAT] is a parasitic disease caused by Trypanosoma spp. and is often referred to as “sleeping sickness”.

Instructions for use

  • This checklist contains the material needed for the different available tests.
  • Do not forget the material needed for sample collection and preparation (venous blood, CSF, lymph node aspirate).

Please consult the “Updated laboratory procedures, 2022” available online via the Laboratory working Group sharepoint page: Laboratory Procedures and Resources.

https://msfintl.sharepoint.com/sites/msfintlcommunities/LabWG/SitePages/Laboratory-Manual-page.aspx

For offline access, contact your laboratory advisor.

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CL CATT dilution assay - titration

8 Product(s)
.

Definition

Human african trypanosomiasis [HAT] is a parasitic disease caused by Trypanosoma spp. and is often referred to as “sleeping sickness”.

Instructions for use

  • This checklist contains the material needed for the different available tests.
  • Do not forget the material needed for sample collection and preparation (venous blood, CSF, lymph node aspirate).

Please consult the “Updated laboratory procedures, 2022” available online via the Laboratory working Group sharepoint page: Laboratory Procedures and Resources.

https://msfintl.sharepoint.com/sites/msfintlcommunities/LabWG/SitePages/Laboratory-Manual-page.aspx

For offline access, contact your laboratory advisor.

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CL Lymph node microscopy

3 Product(s)
.

Definition

Human african trypanosomiasis [HAT] is a parasitic disease caused by Trypanosoma spp. and is often referred to as “sleeping sickness”.

Instructions for use

  • This checklist contains the material needed for the different available tests.
  • Do not forget the material needed for sample collection and preparation (venous blood, CSF, lymph node aspirate).

Please consult the “Updated laboratory procedures, 2022” available online via the Laboratory working Group sharepoint page: Laboratory Procedures and Resources.

https://msfintl.sharepoint.com/sites/msfintlcommunities/LabWG/SitePages/Laboratory-Manual-page.aspx

For offline access, contact your laboratory advisor.

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CL WOO test

7 Product(s)
.

Definition

Human african trypanosomiasis [HAT] is a parasitic disease caused by Trypanosoma spp. and is often referred to as “sleeping sickness”.

Instructions for use

  • This checklist contains the material needed for the different available tests.
  • Do not forget the material needed for sample collection and preparation (venous blood, CSF, lymph node aspirate).

Please consult the “Updated laboratory procedures, 2022” available online via the Laboratory working Group sharepoint page: Laboratory Procedures and Resources.

https://msfintl.sharepoint.com/sites/msfintlcommunities/LabWG/SitePages/Laboratory-Manual-page.aspx

For offline access, contact your laboratory advisor.

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CL Mini Anion Exchange Column

12 Product(s)
.

Definition

Human african trypanosomiasis [HAT] is a parasitic disease caused by Trypanosoma spp. and is often referred to as “sleeping sickness”.

Instructions for use

  • This checklist contains the material needed for the different available tests.
  • Do not forget the material needed for sample collection and preparation (venous blood, CSF, lymph node aspirate).

Please consult the “Updated laboratory procedures, 2022” available online via the Laboratory working Group sharepoint page: Laboratory Procedures and Resources.

https://msfintl.sharepoint.com/sites/msfintlcommunities/LabWG/SitePages/Laboratory-Manual-page.aspx

For offline access, contact your laboratory advisor.

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CL Modified simple centrifugation

4 Product(s)
.

Definition

Human african trypanosomiasis [HAT] is a parasitic disease caused by Trypanosoma spp. and is often referred to as “sleeping sickness”.

Instructions for use

  • This checklist contains the material needed for the different available tests.
  • Do not forget the material needed for sample collection and preparation (venous blood, CSF, lymph node aspirate).

Please consult the “Updated laboratory procedures, 2022” available online via the Laboratory working Group sharepoint page: Laboratory Procedures and Resources.

https://msfintl.sharepoint.com/sites/msfintlcommunities/LabWG/SitePages/Laboratory-Manual-page.aspx

For offline access, contact your laboratory advisor.

No Product(s) in your list!

CL Double centrifugation

6 Product(s)
.

Definition

Human african trypanosomiasis [HAT] is a parasitic disease caused by Trypanosoma spp. and is often referred to as “sleeping sickness”.

Instructions for use

  • This checklist contains the material needed for the different available tests.
  • Do not forget the material needed for sample collection and preparation (venous blood, CSF, lymph node aspirate).

Please consult the “Updated laboratory procedures, 2022” available online via the Laboratory working Group sharepoint page: Laboratory Procedures and Resources.

https://msfintl.sharepoint.com/sites/msfintlcommunities/LabWG/SitePages/Laboratory-Manual-page.aspx

For offline access, contact your laboratory advisor.

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CL RPR test - Syphilis

8 Product(s)
.

Used Techniques

RPR [rapid plasma reagin] detects non-specific antibodies in the blood of the patient that may indicate that Treponema pallidum, that causes syphilis, is present.

Instructions for use

  • Checklist for qualitative and semi-quantitative tests.
  • Do not forget material for venous blood collection.

Please consult the “Updated laboratory procedures, 2022” available online via the Laboratory working Group sharepoint page: Laboratory Procedures and Resources.

https://msfintl.sharepoint.com/sites/msfintlcommunities/LabWG/SitePages/Laboratory-Manual-page.aspx

For offline access, contact your laboratory advisor.

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CL Cary-Blair transport sample

5 Product(s)
.

Definition

List of materials needed to send stool samples to reference laboratories

Instructions for use

Please consult the “Updated laboratory procedures, 2022” available online via the Laboratory working Group sharepoint page: Laboratory Procedures and Resources.

https://msfintl.sharepoint.com/sites/msfintlcommunities/LabWG/SitePages/Laboratory-Manual-page.aspx

For offline access, contact your laboratory advisor.

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CL Stool sample - filter paper transport

3 Product(s)
.

Definition

List of materials needed to send stool samples to reference laboratories

Instructions for use

Please consult the “Updated laboratory procedures, 2022” available online via the Laboratory working Group sharepoint page: Laboratory Procedures and Resources.

https://msfintl.sharepoint.com/sites/msfintlcommunities/LabWG/SitePages/Laboratory-Manual-page.aspx

For offline access, contact your laboratory advisor.

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CL CSF - TI inoculation

10 Product(s)
.

Definition

List of necessary items for the collection of CSF and transport to the reference laboratory using transport medium for CSF (Trans Isolate) medium in case of suspicion of a meningitis outbreak in the African meningitis belt [i].

Instructions for use

The Trans Isolate (TI) medium supports the growth and survival of Neisseria meningitides, Streptococcus pneumoniae and Haemophilus influenza. MSF has an agreement with the Norwegian Public Health Institute in Oslo, WHO Collaborating Centre for Reference and Research on Meningococci, to support its role in the surveillance of the evolution of meningitis strains. As part of this agreement, the Norwegian Public Health Institute is providing the TI medium free of charge to MSF for strain identification alone.

In 2023, MSF is only getting 120 bottles of the TI transport medium
from the supplier is the Norwegian Public Health Institute (Oslo).

As communicated internally previously (LWG, December 2018), the TI transport medium coming from Oslo has always been meant to be used ONLY for the purpose of global surveillance, and not for any other purpose. For any other testing purpose, TI transport medium needs to be requested at country level from the national referral laboratory/public health institute or regional WHO representative. Realizing the complexity of this supply, the MSF laboratory working group is investigating possible alternative supply sources, but this has proven complicated in the past and is unlikely to provide solutions in near future.

The pragmatic decision was made that the 120 TI bottles will be distributed equally to 12 different MSF missions within the African meningitis belt , and that in each country they will be prepositioned in 1 coordination office, available for all sections to use for the specific purpose of sending CSF samples to Oslo only.

[i] The 14 countries in the African Meningitis Belt with enhanced surveillance for meningococcal disease include Benin, Burkina Faso, Cameroon, the Central African Republic, Chad, Côte d'Ivoire, the Democratic Republic of the Congo, Ethiopia, Ghana, Mali, Niger, Nigeria, Sudan and Togo

Other remarks for use:

  • Do not forget material for CSF collection
  • Always wear gloves to manipulate samples

Please consult the “Updated laboratory procedures, 2022” available online via the Laboratory working Group sharepoint page: Laboratory Procedures and Resources.

https://msfintl.sharepoint.com/sites/msfintlcommunities/LabWG/SitePages/Laboratory-Manual-page.aspx

For offline access, contact your laboratory advisor.

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CL GRAM staining

13 Product(s)
.

Used Techniques

Gram staining to differentiate gram-positive and gram-negative bacteria, observe size and morphology using microscopy.

Instructions for use

  • Do not forget the material for sample collection
  • Always wear gloves to manipulate sample

Please consult the “Updated laboratory procedures, 2022” available online via the Laboratory working Group sharepoint page: Laboratory Procedures and Resources.

https://msfintl.sharepoint.com/sites/msfintlcommunities/LabWG/SitePages/Laboratory-Manual-page.aspx

For offline access, contact your laboratory advisor.

No Product(s) in your list!

CL CSF - TI transport

4 Product(s)
.

Definition

List of necessary items for the collection of CSF and transport to the reference laboratory using transport medium for CSF (Trans Isolate) medium in case of suspicion of a meningitis outbreak in the African meningitis belt [i].

Instructions for use

The Trans Isolate (TI) medium supports the growth and survival of Neisseria meningitides, Streptococcus pneumoniae and Haemophilus influenza. MSF has an agreement with the Norwegian Public Health Institute in Oslo, WHO Collaborating Centre for Reference and Research on Meningococci, to support its role in the surveillance of the evolution of meningitis strains. As part of this agreement, the Norwegian Public Health Institute is providing the TI medium free of charge to MSF for strain identification alone.

In 2023, MSF is only getting 120 bottles of the TI transport medium
from the supplier is the Norwegian Public Health Institute (Oslo).

As communicated internally previously (LWG, December 2018), the TI transport medium coming from Oslo has always been meant to be used ONLY for the purpose of global surveillance, and not for any other purpose. For any other testing purpose, TI transport medium needs to be requested at country level from the national referral laboratory/public health institute or regional WHO representative. Realizing the complexity of this supply, the MSF laboratory working group is investigating possible alternative supply sources, but this has proven complicated in the past and is unlikely to provide solutions in near future.

The pragmatic decision was made that the 120 TI bottles will be distributed equally to 12 different MSF missions within the African meningitis belt , and that in each country they will be prepositioned in 1 coordination office, available for all sections to use for the specific purpose of sending CSF samples to Oslo only.

[i] The 14 countries in the African Meningitis Belt with enhanced surveillance for meningococcal disease include Benin, Burkina Faso, Cameroon, the Central African Republic, Chad, Côte d'Ivoire, the Democratic Republic of the Congo, Ethiopia, Ghana, Mali, Niger, Nigeria, Sudan and Togo

Other remarks for use:

  • Do not forget material for CSF collection
  • Always wear gloves to manipulate samples

Please consult the “Updated laboratory procedures, 2022” available online via the Laboratory working Group sharepoint page: Laboratory Procedures and Resources.

https://msfintl.sharepoint.com/sites/msfintlcommunities/LabWG/SitePages/Laboratory-Manual-page.aspx

For offline access, contact your laboratory advisor.

No Product(s) in your list!

Associated module - TI

1 Product(s)
.

Definition

List of necessary items for the collection of CSF and transport to the reference laboratory using transport medium for CSF (Trans Isolate) medium in case of suspicion of a meningitis outbreak in the African meningitis belt [i].

Instructions for use

The Trans Isolate (TI) medium supports the growth and survival of Neisseria meningitides, Streptococcus pneumoniae and Haemophilus influenza. MSF has an agreement with the Norwegian Public Health Institute in Oslo, WHO Collaborating Centre for Reference and Research on Meningococci, to support its role in the surveillance of the evolution of meningitis strains. As part of this agreement, the Norwegian Public Health Institute is providing the TI medium free of charge to MSF for strain identification alone.

In 2023, MSF is only getting 120 bottles of the TI transport medium
from the supplier is the Norwegian Public Health Institute (Oslo).

As communicated internally previously (LWG, December 2018), the TI transport medium coming from Oslo has always been meant to be used ONLY for the purpose of global surveillance, and not for any other purpose. For any other testing purpose, TI transport medium needs to be requested at country level from the national referral laboratory/public health institute or regional WHO representative. Realizing the complexity of this supply, the MSF laboratory working group is investigating possible alternative supply sources, but this has proven complicated in the past and is unlikely to provide solutions in near future.

The pragmatic decision was made that the 120 TI bottles will be distributed equally to 12 different MSF missions within the African meningitis belt , and that in each country they will be prepositioned in 1 coordination office, available for all sections to use for the specific purpose of sending CSF samples to Oslo only.

[i] The 14 countries in the African Meningitis Belt with enhanced surveillance for meningococcal disease include Benin, Burkina Faso, Cameroon, the Central African Republic, Chad, Côte d'Ivoire, the Democratic Republic of the Congo, Ethiopia, Ghana, Mali, Niger, Nigeria, Sudan and Togo

Other remarks for use:

  • Do not forget material for CSF collection
  • Always wear gloves to manipulate samples

Please consult the “Updated laboratory procedures, 2022” available online via the Laboratory working Group sharepoint page: Laboratory Procedures and Resources.

https://msfintl.sharepoint.com/sites/msfintlcommunities/LabWG/SitePages/Laboratory-Manual-page.aspx

For offline access, contact your laboratory advisor.

No Product(s) in your list!

Associated module - CSF

1 Product(s)
.

Used Techniques

Rapid test for the detection of bacteria responsible for meningitis in CSF (cerebro spinal fluid).

Detects the meningococcus A, B, C, Y/W135, the streptococcus B and pneumoniae, as well as the Haemophilus influenzae B.

Instructions for use

  • Do not forget material for lumbar puncture

Please consult the “Updated laboratory procedures, 2022” available online via the Laboratory working Group sharepoint page: Laboratory Procedures and Resources.

https://msfintl.sharepoint.com/sites/msfintlcommunities/LabWG/SitePages/Laboratory-Manual-page.aspx

For offline access, contact your laboratory advisor.

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Associated module - ZN

1 Product(s)
.

Used Techniques

Smears stained with Ziehl-Neelsen for microscopic detection of mycobacteria.

Instructions for use

Wearing a mask is mandatory during the preparation of the smear. It is also mandatory to wear gloves for any handling of samples.

CAUTION: never pipet by mouth!

The rack for slides can be manufactured locally.

Other possible staining: fluorescence system with auramine staining. Contact your laboratory referent for more information on performing auramine staining.

NB: The TB culture checklists are in the TB culture catalogue.

Please consult the “Updated laboratory procedures, 2022” available online via the Laboratory working Group sharepoint page: Laboratory Procedures and Resources.

https://msfintl.sharepoint.com/sites/msfintlcommunities/LabWG/SitePages/Laboratory-Manual-page.aspx

For offline access, contact your laboratory advisor.

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Associated modules - transfusion

2 Product(s)
.

Precatalog Text (English)

The 5 reagents for blood grouping (anti-AB, anti-A, anti-B, anti Rh D, Rhesus negative control) must all be used and come from the same manufacturer

Definition

ABO reagents contain immunoglobulins used for the blood group typing by determining the presence or absence of antigens on the red cells.

Instructions for use

Do not forget equipment for blood collection (venous or capillary)

It is recommended to wear gloves for any handling of blood sample

No Product(s) in your list!

Associated module - sample collection

1 Product(s)
.

Used Techniques

Venipuncture in vacuum sterile tubes.

There is an associated module with all material needed for safety blood sampling, in context of haemorragic fever.

Instructions for use

Closed system to prevent any contact with blood.

It is recommended to wear gloves for any handling of blood samples.

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PRODUCT INFO Quantity
1

Associated module - DBS

1 Product(s)
.

Used Techniques

Capillary blood collection with lancets, with (or without) microtainer tubes.

Instructions for use

It is recommended to wear gloves for any handling of blood samples.

Different lancets available:

  • STSSLANCSAM2: SAFETY LANCET, medium flow, needle 21G x 1.8mm, green, s.u.
    • Use: Rapid tests and handheld analysers in infants, children and adults.
    • Blood volume: 10-50µl.
    • Puncture site: heel (up to 6 months ) finger
  • STSSLANCSH3: SAFETY LANCET, high flow, blade 1.5 X 1.2mm, pink, s.u.
    • Use: For DBS for HIV EID and VL in infants, children and adults.
    • Blood volume: 50-250µl.
    • Puncture site: heel (up to 6 months) finger
  • STSSLANCSHL1 - SAFETY LANCET, low flow, needle 28Gx1.5mm, light blue, s.u.
    • Use: rapid tests and handheld analysers in infants and children.
    • Blood volume: 10-20µl.
    • Puncture site: heel (up to 6 months) finger
No Product(s) in your list!
PRODUCT INFO Quantity
1

Associated modules - reagents and equipment

2 Product(s)
.

Used Techniques

Haemoglobin photometer: HemoCue Hb 301 (tropicalized model)

Instructions for use

Use only microcuvettes which are designed for the photometer. They are different for the Hb 301 and the Hb 201+ models.

Do not forget equipment for blood collection (capillary or venous)

It is recommended to wear gloves for any handling of blood samples.

Please consult the “Updated laboratory procedures, 2022” available online via the Laboratory working Group sharepoint page: Laboratory Procedures and Resources.

https://msfintl.sharepoint.com/sites/msfintlcommunities/LabWG/SitePages/Laboratory-Manual-page.aspx

For offline access, contact your laboratory advisor.

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Associated modules - malaria

2 Product(s)
.

Used Techniques

Thin / thick blood film stained with Giemsa for the detection of malaria, borreliasis, leishmaniasis, filariasis.

Instructions for use

The Giemsa solution should be extemporaneously prepared. The racks for staining and drying slides can be locally manufactured.

Malaria, filariasis and borreliasis are generally done with a capillary blood sample (we can also use venous blood). Do not forget equipment for blood sample collection.

It is recommended to wear gloves for any handling of blood samples.

Leishmaniasis needs an lymph node or spleen puncture, what requires a particular training which is organized only in some specific programmes.

Visceral leishmaniasis (Kala-Azar) requires in addition another diagnostic method with a serological test.

Please consult the “Updated laboratory procedures, 2022” available online via the Laboratory working Group sharepoint page: Laboratory Procedures and Resources.

https://msfintl.sharepoint.com/sites/msfintlcommunities/LabWG/SitePages/Laboratory-Manual-page.aspx

For offline access, contact your laboratory advisor.

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CL TB Lam

5 Product(s)
.

Definition

List of needed articles, used for TB Lam tests

Instructions for use

It is recommended to wear gloves for any handling of urine samples.

Please consult the “Updated laboratory procedures, 2022” available online via the Laboratory working Group sharepoint page: Laboratory Procedures and Resources.

https://msfintl.sharepoint.com/sites/msfintlcommunities/LabWG/SitePages/Laboratory-Manual-page.aspx

For offline access, contact your laboratory advisor.

No Product(s) in your list!