ILAB - Laboratory checklists

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CL Laboratory safety and infection control

23 Product(s)
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Definition

List of essential articles to guarantee safety and infection control in a laboratory.

Instructions for use

A safety cabinet is only recommended in certain situations. Contact your laboratory referent at headquarters for further information.

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CL Blood collection venipuncture

19 Product(s)
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Used Techniques

Venipuncture in vacuum sterile tubes.

There is an associated module with all material needed for safety blood sampling, in context of haemorragic fever.

Instructions for use

Closed system to prevent any contact with blood.

It is recommended to wear gloves for any handling of blood samples.

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CL Haemoglobin

11 Product(s)
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Used Techniques

Haemoglobin photometer: HemoCue Hb 301 (tropicalized model)

Instructions for use

Use only microcuvettes which are designed for the photometer. They are different for the Hb 301 and the Hb 201+ models.

Do not forget equipment for blood collection (capillary or venous)

It is recommended to wear gloves for any handling of blood samples.

Please consult the “Updated laboratory procedures, 2022” available online via the Laboratory working Group sharepoint page: Laboratory Procedures and Resources.

https://msfintl.sharepoint.com/sites/msfintlcommunities/LabWG/SitePages/Laboratory-Manual-page.aspx

For offline access, contact your laboratory advisor.

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CL haematocrit

7 Product(s)
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Used Techniques

Determination of haematocrit with electrical centrifuge.

Instructions for use

Capillary or venous sample in EDTA tube. Do not forget equipment for blood collection.

Used for concentration techniques in trypanosomiasis programmes.

The haematocrit capillary tubes should be sealed before centrifuging with sealing paste.

It is recommended to wear gloves for any handling of blood samples.

Please consult the “Updated laboratory procedures, 2022” available online via the Laboratory working Group sharepoint page: Laboratory Procedures and Resources.

https://msfintl.sharepoint.com/sites/msfintlcommunities/LabWG/SitePages/Laboratory-Manual-page.aspx

For offline access, contact your laboratory advisor.

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CL Cryptococcus, CSF, Indian ink

9 Product(s)
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Used Techniques

Examination of CSF:

  • Using India ink for cryptococcus detection.
  • Rapid immunochromatographic test (possible also on serum, plasma or whole blood) with possible qualitative and semi-quantitative titration procedures‌ for cryptococcus.
  • Pandy test (detection of increased levels of globulin in CSF).

Instructions for use

It is strongly recommended to use an electrical centrifuge.

Do not forget the material and equipment for sample collection.

It is recommended to wear gloves for any handling of blood samples.

Please consult the “Updated laboratory procedures, 2022” available online via the Laboratory working Group sharepoint page: Laboratory Procedures and Resources.

https://msfintl.sharepoint.com/sites/msfintlcommunities/LabWG/SitePages/Laboratory-Manual-page.aspx

For offline access, contact your laboratory advisor.

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CL cryptococcus, rapid test

11 Product(s)
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Used Techniques

Examination of CSF:

  • Using India ink for cryptococcus detection.
  • Rapid immunochromatographic test (possible also on serum, plasma or whole blood) with possible qualitative and semi-quantitative titration procedures‌ for cryptococcus.
  • Pandy test (detection of increased levels of globulin in CSF).

Instructions for use

It is strongly recommended to use an electrical centrifuge.

Do not forget the material and equipment for sample collection.

It is recommended to wear gloves for any handling of blood samples.

Please consult the “Updated laboratory procedures, 2022” available online via the Laboratory working Group sharepoint page: Laboratory Procedures and Resources.

https://msfintl.sharepoint.com/sites/msfintlcommunities/LabWG/SitePages/Laboratory-Manual-page.aspx

For offline access, contact your laboratory advisor.

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CL Test trypanosomiasis (CATT)

16 Product(s)
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Used Techniques

Rapid serological test (CATT test) used for the screening of Trypanosoma gambiense (sleeping sickness).

Instructions for use

The CATT is the only screening test for trypanosomiasis. The diagnosis of trypanosomiasis must be completed with other tests:

  • liquid of ganglionary puncture
  • Woo test: looking for trypanosoma in a blood sample
  • lumbar puncture (double centrifuge method) to determine the stage of the disease

This list includes the equipment needed for the CATT test on whole blood and the CATT test dilution. Do not forget equipment for sample collection.

It is recommended to wear gloves for any handling of blood samples.

Please consult the “Updated laboratory procedures, 2022” available online via the Laboratory working Group sharepoint page: Laboratory Procedures and Resources.

https://msfintl.sharepoint.com/sites/msfintlcommunities/LabWG/SitePages/Laboratory-Manual-page.aspx

For offline access, contact your laboratory advisor.

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CL Kala-Azar test (DAT)

26 Product(s)
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Used Techniques

Rapid serological test (DAT) used for the detection of visceral leishmaniasis (Kala-Azar) in serum or whole blood.

Instructions for use

Do not forget equipment for blood sample collection (capillary or venous).

It is recommended to wear gloves for any handling of blood samples.

Please consult the “Updated laboratory procedures, 2022” available online via the Laboratory working Group sharepoint page: Laboratory Procedures and Resources.

https://msfintl.sharepoint.com/sites/msfintlcommunities/LabWG/SitePages/Laboratory-Manual-page.aspx

For offline access, contact your laboratory advisor.

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CL stain malaria, filariasis, leishmaniasis

24 Product(s)
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Used Techniques

Thin / thick blood film stained with Giemsa for the detection of malaria, borreliasis, leishmaniasis, filariasis.

Instructions for use

The Giemsa solution should be extemporaneously prepared. The racks for staining and drying slides can be locally manufactured.

Malaria, filariasis and borreliasis are generally done with a capillary blood sample (we can also use venous blood). Do not forget equipment for blood sample collection.

It is recommended to wear gloves for any handling of blood samples.

Leishmaniasis needs an lymph node or spleen puncture, what requires a particular training which is organized only in some specific programmes.

Visceral leishmaniasis (Kala-Azar) requires in addition another diagnostic method with a serological test.

Please consult the “Updated laboratory procedures, 2022” available online via the Laboratory working Group sharepoint page: Laboratory Procedures and Resources.

https://msfintl.sharepoint.com/sites/msfintlcommunities/LabWG/SitePages/Laboratory-Manual-page.aspx

For offline access, contact your laboratory advisor.

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CL skin microfilariasis (onchocerciasis)

13 Product(s)
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Used Techniques

Detection of onchocerciasis on bloodless skin biopsy.

Instructions for use

For the sodium chloride solution, use a normal saline infusion instead of the powder to be diluted (need of a spatula, measuring cylinder and scale).

It is recommended to wear gloves for any handling of samples.

Please consult the “Updated laboratory procedures, 2022” available online via the Laboratory working Group sharepoint page: Laboratory Procedures and Resources.

https://msfintl.sharepoint.com/sites/msfintlcommunities/LabWG/SitePages/Laboratory-Manual-page.aspx

For offline access, contact your laboratory advisor.

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CL drepanocytosis, Emmel test

14 Product(s)
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Used Techniques

Emmel test (= sickle cell test) on whole blood/ capillary blood with sodium disulfite (metabisulfite) or saline.

Instructions for use

The technique with sodium disulfite is recommended as it is more sensitive than saline.

A scale is needed to prepare the reagent (2% solution: 0.5 g for 25 ml distilled water).

The metabisulphite solution is unstable and can be kept maximum 8 hours.

Alternative: test with paraffine.

It is recommended to wear gloves during the test.

See also the checklist for blood collection/venipuncture for the necessary material for sampling.

Please consult the “Updated laboratory procedures, 2022” available online via the Laboratory working Group sharepoint page: Laboratory Procedures and Resources.

https://msfintl.sharepoint.com/sites/msfintlcommunities/LabWG/SitePages/Laboratory-Manual-page.aspx

For offline access, contact your laboratory advisor.

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CL CSF, proteins, Pandy test

11 Product(s)
.

Used Techniques

Examination of CSF:

  • Using India ink for cryptococcus detection.
  • Rapid immunochromatographic test (possible also on serum, plasma or whole blood) with possible qualitative and semi-quantitative titration procedures‌ for cryptococcus.
  • Pandy test (detection of increased levels of globulin in CSF).

Instructions for use

It is strongly recommended to use an electrical centrifuge.

Do not forget the material and equipment for sample collection.

It is recommended to wear gloves for any handling of blood samples.

Please consult the “Updated laboratory procedures, 2022” available online via the Laboratory working Group sharepoint page: Laboratory Procedures and Resources.

https://msfintl.sharepoint.com/sites/msfintlcommunities/LabWG/SitePages/Laboratory-Manual-page.aspx

For offline access, contact your laboratory advisor.

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CL TB, microscopy sputum examination Ziehl Neelsen

40 Product(s)
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Used Techniques

Smears stained with Ziehl-Neelsen for microscopic detection of mycobacteria.

Instructions for use

Wearing a mask is mandatory during the preparation of the smear. It is also mandatory to wear gloves for any handling of samples.

CAUTION: never pipet by mouth!

The rack for slides can be manufactured locally.

Other possible staining: fluorescence system with auramine staining. Contact your laboratory referent for more information on performing auramine staining.

NB: The TB culture checklists are in the TB culture catalogue.

Please consult the “Updated laboratory procedures, 2022” available online via the Laboratory working Group sharepoint page: Laboratory Procedures and Resources.

https://msfintl.sharepoint.com/sites/msfintlcommunities/LabWG/SitePages/Laboratory-Manual-page.aspx

For offline access, contact your laboratory advisor.

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CL TB, microscopy sputum exam. Auramine

24 Product(s)
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Used Techniques

Staining technique using the fluorescent auramine reagent for microscopic detection of mycobacteria.

Instructions for use

Wearing a mask is mandatory during the preparation of the smear. It is also mandatory to wear gloves for any handling of samples.

CAUTION: never pipet by mouth!

The rack for slides can be manufactured locally.

Other possible staining: staining with Ziehl Neelsen. Contact your laboratory referent for more information.

NB: The TB culture checklists are in the TB culture catalogue.

Please consult the “Updated laboratory procedures, 2022” available online via the Laboratory working Group sharepoint page: Laboratory Procedures and Resources.

https://msfintl.sharepoint.com/sites/msfintlcommunities/LabWG/SitePages/Laboratory-Manual-page.aspx

For offline access, contact your laboratory advisor.

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CL parasitology of stools

18 Product(s)
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Used Techniques

Direct stools examination.

Instructions for use

For lack of stools sample containers, use urine sample containers [ELAECONT6U-].

Please consult the “Updated laboratory procedures, 2022” available online via the Laboratory working Group sharepoint page: Laboratory Procedures and Resources.

https://msfintl.sharepoint.com/sites/msfintlcommunities/LabWG/SitePages/Laboratory-Manual-page.aspx

For offline access, contact your laboratory advisor.

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CL examination of urine, biochemistry

5 Product(s)
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Used Techniques

  • Biochemistry : urine strips: glucose, proteins, pH, density, ketones, blood, nitrites, leucocytes.
  • Direct examination of urine sediment for cell count and detection of parasites (trichomonas, bilharzia).

Instructions for use

Two different strips are available; with 2 and 8 parameters (see checklist). Select the strips according to the parameters to be determined.

Please consult the “Updated laboratory procedures, 2022” available online via the Laboratory working Group sharepoint page: Laboratory Procedures and Resources.

https://msfintl.sharepoint.com/sites/msfintlcommunities/LabWG/SitePages/Laboratory-Manual-page.aspx

For offline access, contact your laboratory advisor.

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CL exam. of urine, cell count + sediment

12 Product(s)
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Used Techniques

  • Biochemistry : urine strips: glucose, proteins, pH, density, ketones, blood, nitrites, leucocytes.
  • Direct examination of urine sediment for cell count and detection of parasites (trichomonas, bilharzia).

Instructions for use

Two different strips are available; with 2 and 8 parameters (see checklist). Select the strips according to the parameters to be determined.

Please consult the “Updated laboratory procedures, 2022” available online via the Laboratory working Group sharepoint page: Laboratory Procedures and Resources.

https://msfintl.sharepoint.com/sites/msfintlcommunities/LabWG/SitePages/Laboratory-Manual-page.aspx

For offline access, contact your laboratory advisor.

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CL clinical chemistry analyzer i-STAT

25 Product(s)
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Definition

The i-STAT is a handheld device for the automated, quantitative determination of clinical chemistry parameters using cartridges for single use. Cartridges with a different configuration panels for the analysis of blood gases, electrolytes, coagulation, hematology, glucose and creatinine are available.

Instructions for use

Quality control cartridges should be used at least once daily.

Do not forget equipment for blood sample collection (capillary, venous, arterial).

It is recommended to wear gloves for any handling of blood samples.

Please consult the “Updated laboratory procedures, 2022” available online via the Laboratory working Group sharepoint page: Laboratory Procedures and Resources.

https://msfintl.sharepoint.com/sites/msfintlcommunities/LabWG/SitePages/Laboratory-Manual-page.aspx

For offline access, contact your laboratory advisor.

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CL CD4 analyzer PIMA (Abbott)

13 Product(s)
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Precatalog Text (English)

The PIMA CD4 analyzer has been discontinued by the manufacturer in May 2022.

The consumables and spare parts remain available. Contact your laboratory advisor for alternative options.

Definition

PIMA analyser is a benchtop and portable fixed-volume cytometer for the enumeration of CD4+ T cells with PIMA CD4 test cartridge on capillary or venous blood.

Instructions for use

Do not forget the equipment for blood sampling with EDTA tube (see checklist blood collection).

If the test is performed on capillary blood, the ordering of the sample collection kit is mandatory, because it contains specific lancets for PIMA CD4 use.

Please consult the “Updated laboratory procedures, 2022” available online via the Laboratory working Group sharepoint page: Laboratory Procedures and Resources.

https://msfintl.sharepoint.com/sites/msfintlcommunities/LabWG/SitePages/Laboratory-Manual-page.aspx

For offline access, contact your laboratory advisor.

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CL CD4 FACSCount® (Becton Dickinson)

26 Product(s)
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Precatalog Text (English)

BD FacsCount CD4 analyzer have been discontinued by the manufacturer. Contact your laboratory advisor for alternative options.

The reagents and consumables for the FacsCount remain available up to end of 2023.

Definition

BD FACSCount is a flow cytometer for the enumeration of CD4+ (and CD3+, CD8+) T cells and the measurement of the percentage of CD4+ T cells among all lymphocytes in blood.

Instructions for use

Do not forget the equipment for blood sampling with EDTA tube (see checklist blood collection)

Please consult the “Updated laboratory procedures, 2022” available online via the Laboratory working Group sharepoint page: Laboratory Procedures and Resources.

https://msfintl.sharepoint.com/sites/msfintlcommunities/LabWG/SitePages/Laboratory-Manual-page.aspx

For offline access, contact your laboratory advisor.

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CL ELISA reader for TSH monitoring

20 Product(s)
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Definition

Enzyme-linked immunosorbent assay (ELISA) method to qualitatively and/or quantitatively determine the presence of a protein through absorbance using a microplate reader.
This reader can be used to measure absorbance with multiple ELISA plate formats.

In MSF, the ELISA test is currently used to monitor thyroid stimulating hormone (TSH) in MDR TB patients, in high case loads of some serological tests and for operational research.

Instructions for use

The ELISA washer is reserved for programmes with high ELISA analysis throughput (many plates) and good infrastructure.

Do not forget equipment for venous blood sample collection.

Please consult the “Updated laboratory procedures, 2022” available online via the Laboratory working Group sharepoint page: Laboratory Procedures and Resources.

https://msfintl.sharepoint.com/sites/msfintlcommunities/LabWG/SitePages/Laboratory-Manual-page.aspx

For offline access, contact your laboratory advisor.

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CL haematology analyzer SYSMEX XP300/XQ320

15 Product(s)
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Precatalog Text (English)

The haematology analyzer XQ-320 replaces the XP-300 starting from 2023. The XQ-320 uses the same reagents and solutions as the XP-300 but spare parts are different for both models.

Definition

Haematology analysers are used for full blood counts: total white blood cell count, red blood cell count and platelets. In addition, the analyser measures also haemoglobin, haematocrit, percentage of lymphocytes and granulated white blood cells.

Instructions for use

Do not forget the equipment for blood sampling with EDTA tubes (see checklist blood collection).

Please consult the “Updated laboratory procedures, 2022” available online via the Laboratory working Group sharepoint page: Laboratory Procedures and Resources.

https://msfintl.sharepoint.com/sites/msfintlcommunities/LabWG/SitePages/Laboratory-Manual-page.aspx

For offline access, contact your laboratory advisor.

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CL Molecular biology GeneXpert GX-IV MTB

13 Product(s)
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Precatalog Text (English)

The MTB/RIF cartridge will be discontinued by the manufacturer in December 2023 and replaced by the MTB/RIF Ultra cartridge.

Definition

The GeneXpert is an automated molecular cartridge based, closed system to perform nucleic acid amplification testing (NAAT).

The test Xpert MTB/RIF and MTB/RIF Ultra cartridge is used to detect Mycobacterium tuberculosis and rifampicine resistance. The MTB/RIF Ultra has a lower limit of detection than MTB/RIF, reduces false rifampicin resistance and better differentiate silent from resistant-conferring mutations.

The test Xpert MTB/XDR is used to detect extensively drug resistant Mycobacterium tuberculosis, with resistance detection to isoniazid, ethionamide, fluoroquinolone, second line injectable drug.

Instructions for use

The GeneXpert® MTB test does not eliminate the need for conventional microscopy, culture and DST, which are required to monitor treatment progress and to detect resistance of Mycobacterium tuberculosis to drugs.

The operating temperature for GeneXpert device is 15-30°C.

Please consult the “Updated laboratory procedures, 2022” available online via the Laboratory working Group sharepoint page: Laboratory Procedures and Resources.

https://msfintl.sharepoint.com/sites/msfintlcommunities/LabWG/SitePages/Laboratory-Manual-page.aspx

For offline access, contact your laboratory advisor.

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CL Clin. chemistry spectrophotometer Humalyzer 2000 (Human)

45 Product(s)
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Precatalog Text (English)

The Humalyzer 2000 has been stopped by the manufacturer. Contact your laboratory advisor for alternative options.

The support for Humalyzer 2000 will be discontinued in November 2023, and spare parts for this instrument will only be available as long as stock lasts.

Definition

A biochemistry analyser (commonly called "spectrophotometer") is used to carry out different clinical and haematology analyses such as liver function test (ALAT, ASAT), renal function test (creatinine, bilirubine) etc.

Instructions for use

Specific kits allow to carry out each analysis. It is compulsory to use the controls.

The dosage kits and control solutions are thermosensitive items: they must be transported by cold chain and kept refrigerated between 2 and 8°C.

Do not forget equipment for venous blood sample collection.

Please consult the “Updated laboratory procedures, 2022” available online via the Laboratory working Group sharepoint page: Laboratory Procedures and Resources.

https://msfintl.sharepoint.com/sites/msfintlcommunities/LabWG/SitePages/Laboratory-Manual-page.aspx

For offline access, contact your laboratory advisor.

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STD STSSBSVT5H