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4-NITROBENZOIC ACID (PNB), 25 g, powder, vial

https://unicat.msf.org/cat/product/18819
STD SLASNIBE25P

Valid Article

Former Code(s): SLASNIBE025
P
The order of this product needs to be justified and is only acceptable under certain conditions.
1525
Thermosensitive codes are defined for storage and transportation temperature requirements of the products.
OC subscriptions: included in supply or field order tools (e.g. UniField)
SC subscriptions: included in supply or field tools
OC validations: approved for procurement and use by an OC for international or local orders specifying context and activity in Medical Standard Lists (MSL)

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4-NITROBENZOIC ACID (PNB)

Reserved for TB culture programmes.

Definition

This reagent is added to TLA or LJ medium for primary identification of Mycobacterium tuberculosis complex.

Synonym

p-Nitrobenzoic acid, PNB

Specifications

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Technical specifications

  • CAS n°: 62-23-7
  • Powder
  • Formula: C7H5NO4
  • Purity: 98%
  • Quality: pure

Packaging & Labelling

  • 25 g in bottle
  • Transport not regulated

Instructions for use

​This reagent is used to identify M. tuberculosis complex (MTBc). The principle is that MTBc is inhibited by ρ-Nitrobenzoic
acid (PNB), whereas non-tuberculous mycobacteria
(NTM) are resistant. Primary identification is done by the comparison of growth among the two media.

PNB is added to Middlebrook 7H11 or Lowenstein-Jensen during medium preparation. Follow Standard Operational Procedures for stock solution preparation and plates/tubes preparation.

Storage

  • At room temperature
  • Keep tightly closed
  • 4- nitrobenzoic acid solution should be used immediately, or stored in the refrigerator.

Classification EC Regulation N° 1272/2008

  • Acute toxicity, Oral: Category 4, H302
  • Serious eye damage: Category 1, H319

Signal Word

Danger

Image Image
H302Harmful if swallowed
H315Causes skin irritation
H319Causes serious eye irritation
H335May cause respiratory irritation
P280Wear protective gloves/protective clothing/eye protection/face protection
P301 + P312IF SWALLOWED: call a POISON CENTER or doctor/physician if you feel
P305 + P351 + P338IF IN EYES: rinse cautiously with water for several minutes. If the victim wears contact lenses, remove them if possible. Keep rinsing

MSF requirements

ρ-Nitrobenzoic acid (PNB) is used in TLA and LJ cultures for the​ primary identification of Mycobacterium tuberculosis (MTBc) against non-tuberculous mycobacteria (NMT).

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