(API System) NH STRIP Neisseria & Haemophilus [BMX-10400]
Valid Article
API NH STRIP, Neisseria & Haemophilus
CAUTION
This test is worded and codified by unit to make the order easier, but it is always packaged in kit of 10.
Definition
API NH is a standardized system for the identification of Neisseria, Haemophilus (and related genera) and Moraxella catarrhalis (Branhamella catarrhalis), which uses miniaturized tests, as well as a specially adapted database. The API NH strips enables the biotyping of Haemophilus influenzae and Haemophilus parainfluenzae, as well as the detection of a penicillinase.
Inoculation and reading are done manually, and identification is done with the software.
Specifications
Components
R1 ZYM B: contains methanol
Kit containing:
- 10 API NH strips
- 10 ampules of API NaCl 0.85 % Medium (2 ml)
- 1 ampule of JAMES reagent (R1 - 5 ml) solvent + 1 bottle of JAMES (R2 - 0.033 g) reagent
- 1 ampule of ZYM B (R1 - 5 ml) solvent + 1 bottle of ZYM B (R2 - 0.007 g) reagent
- 10 incubation boxes
- 10 result sheets
- 1 package insert provided in the kit.
Technical specifications
Strips consisting of 10 microtubes containing dehydrated substrates which enable the performance of 12 identification tests (enzymatic reactions or sugar fermentations), as well as the detection of a penicillinase.
The colours changes occur during incubation, due to metabolism, spontaneously or by addition of reagents.
- R1 James: clear colorless or nearly colorless liquid
- R2 James: solid off-white powder
- R1 ZYM B: clear colorless or nearly colorless liquid
- R2 ZYM B: solid off-white powder
- ZYM B after reconstitution is yellow to amber
Packaging & Labelling
Kit of 10 strips and associated reagents
Transport Dangerous Goods
Zym B - R1 :
- UN1992
- Class: 3 (6.1)
- Packing group: III
- Proper shipping name: Flammable liquid, toxic, n.o.s (Methanol Solution)
James - R1:
- UN1789
- Class: 8
- Packing group: III
- Proper shipping name: Hydrochloric acid.
Chemical kit:
- UN3316
- Class: 9
- Packing group: III
- Proper shipping name: Chemical kit
To be Ordered Separately
- Mineral oil
- McFarland Standard, No. 4 on the scale
- Swabs
- Pipettes or PSIpettes
- Ampule rack
- Ampule protector
- General microbiology laboratory equipment.
- ATB™ NEW and APIWEB™ software for identification
Instructions for use
Follow the instructions of the package insert
Please consult the “Bacteriology laboratory procedures and resources” available online via the Laboratory working Group sharepoint page: Laboratory Procedures and Resources
For offline access, contact your laboratory advisor
Precautions for Use
All biological samples must be considered as potentially infectious and handled with the usual precautions (compulsory wearing of gloves, hand washing, etc.).
Storage
- Shelf life: 11 months.
- Guaranteed minimum remaining shelf life at delivery: 1/3 of the total shelf life.
Strips:
- Store between 2°C and 8°C.
- Do not use after expiry date.
Media:
- Store between 2°C and 30°C.
- Do not use after expiry date.
Reagents:
- Store between 2°C and 8°C.
- Store in the dark. Wrap the bottles of JAMES reagents in aluminium foil.
- The JAMES reagent may be kept up to 1 month after the ampules have been opened and the reagents reconstituted.
- The ZYM B reagent may be kept up to 2 weeks after the ampules have been opened and the reagents reconstituted.
- Make sure that the reagents are put back in the fridge immediately after use.
Waste management
After use, sterilize the cultures and all contaminated material.
Contains substances with hazardous and carcinogenic characteristics. Please contact your watsan referent for advice on proper disposal.
Detailed hazard and precautionary information can be found in the safety data sheet (SDS).
Classification EC Regulation N° 1272/2008
JAMES - R1:
- Skin corrosion/irritation, category 1, H314
- Serious eye damage/eye irritation, category 1, H318
ZYM B - R1:
- Flammable liquids, Category 3, H226
- Acute toxicity, oral, Category 4, H302
- Acute toxicity, dermal, Category 3, H311
- Acute toxicity, inhalation, Category 3, H331
- Specific target organ toxicity - single exposure, Category 1, H370
Signal Word
(James R1 and James R2)
Danger - R1
Warning - R2
H314 | Causes severe skin burns and eye damage. |
H315 | Causes skin irritation |
H318 | Causes serious eye damage. |
H319 | Causes serious eye irritation |
H335 | May cause respiratory irritation |
P260 | Do not breathe dust/fume/gas/mist/vapours/spray. |
P264 | Wash … thoroughly after handling |
P280 | Wear protective gloves/protective clothing/eye protection/face protection |
P310 | Immediately call a POISON CENTER or doctor/physician. |
P363 | Wash contaminated clothing before reuse. |
P301 + P330 + P331 | IF SWALLOWED: rinse mouth. Do NOT induce vomiting |
P302 + P352 | IF ON SKIN: wash with plenty of soap and water |
P303 + P361 + P353 | IF ON SKIN (or hair): Remove/Take off immediately all contaminated clothing. |
P304 + P340 | IF INHALED: Remove to fresh air and keep at rest in a position comfortable for breathing. |
P305 + P351 + P338 | IF IN EYES: rinse cautiously with water for several minutes. If the victim wears contact lenses, remove them if possible. Keep rinsing |
P501 | Dispose of contents/container to location in accordance with local and regional/ national/ international regulations |
Signal Word
Danger
H226 | Flammable liquid and vapour. |
H302 | Harmful if swallowed |
H311 | Toxic in contact with skin. |
H331 | Toxic if inhaled. |
H370 | Causes damage to organs (or state all organs affected, if known) (state route of exposure if it is conclusively proven that no other routes of exposure cause the hazard). |
P210 | Keep away from heat/sparks/open flames/hot surfaces. – No smoking. |
P280 | Wear protective gloves/protective clothing/eye protection/face protection |
P301 + P312 | IF SWALLOWED: call a POISON CENTER or doctor/physician if you feel |
P302 + P352 | IF ON SKIN: wash with plenty of soap and water |
P304 + P340 | IF INHALED: Remove to fresh air and keep at rest in a position comfortable for breathing. |
Signal Word
Warning
H302 | Harmful if swallowed |
H351 | Suspected of causing cancer (state route of exposure if it is conclusively proven that no other routs of exposure cause the hazard). |
P280 | Wear protective gloves/protective clothing/eye protection/face protection |
P301 + P312 | IF SWALLOWED: call a POISON CENTER or doctor/physician if you feel |
P308 + P313 | IF exposed or concerned: Get medical advice/attention. |
MSF requirements
Reserved to bacteriology programmes.