UREA INDOL BROTH, 10x10ml [BMX-55752]
Valid Article
UREA INDOL BROTH
Definition
The Urea Indol broth is a medium enabling the detection of the presence of urease or tryptophan deaminase (TDA) and the production of indol in enterobacteria. It thus enables the presumptive differentiation of bacteria possessing these characteristics.
Specifications
Components
Theoretical formula:
- L-Tryptophan 3 g
- Monopotassium phosphate 1 g
- Dipotassium phosphate 1 g
- Sodium chloride 5 g
- Urea 20 g
- Phenol red 0.025 g
- 95% alcohol 0.01 ml
- Purified water 1 L
Technical specifications
Ready to use medium.
Packaging & Labelling
10 x 10 ml bottles.
To be Ordered Separately
- Kovacs' reagent
- Indole TDA ID reagent
- Bacteriology incubator
- Graduated pipettes
- Test tubes
- QC strains
Instructions for use
Allow bottles to come to room temperature.
Please consult the “Bacteriology laboratory procedures and resources” available online via the Laboratory working Group sharepoint page: Laboratory Procedures and Resources https://msfintl.sharepoint.com/sites/msfintlcommunities/LabWG/SitePages/Bacteriology-Laboratory-Resources.aspx
For offline access, contact your laboratory advisor
Precautions for Use
All biological samples must be considered as potentially infectious and handled with the usual precautions (compulsory wearing of gloves, hand washing, etc.).
Storage
- Store between 2°C and 8°C
- Do not use after expiry date
- Shelf life: 16 months
- Guaranteed minimum remaining shelf life at delivery: 1/3 of the total shelf life
Waste management
After use, sterilize the cultures and all contaminated material. Please contact your watsan referent for advice on proper disposal.
MSF requirements
Reserved for bacteriology programmes.