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MIDDLEBROOK 7H10 AGAR, dehydrated 500g [BD-262710]

https://unicat.msf.org/cat/product/73029
STD SBCMAGAR7H10

Valid Article

W0104010101 - Dehydrated culture media (dcm)
European Medical Device Nomenclature (EMDN) is the nomenclature of use by manufacturers when registering their medical devices in the EUDAMED database. EMDN is characterised by its alphanumeric structure that is established in a seven-level hierarchical tree.
P
The order of this product needs to be justified and is only acceptable under certain conditions.
CT30
Thermosensitive codes are defined for storage and transportation temperature requirements of the products.
OC subscriptions: included in supply or field order tools (e.g. UniField)
OC validations: approved for procurement and use by an OC for international or local orders specifying context and activity in Medical Standard Lists (MSL)
SC subscriptions: included in supply or field tools

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MIDDLEBROOK 7H10 Agar, dehydrated

Reserved for TB culture programmes.

Definition

Agar to prepare Middlebrook 7H10 media. Used with glycerol, Middlebrook OADC Enrichment and antibiotic / antimycotic mixture for isolation, cultivation and susceptibility testing of mycobacteria.

Specifications

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Components

Approximate Formula* Per 900 mL

  • Ammonium Sulfate....................................... 0.5 g
  • Monopotassium Phosphate........................... 1.5 g
  • Disodium Phosphate..................................... 1.5 g
  • Sodium Citrate.............................................. 0.4 g
  • Magnesium Sulfate.................................. 25.0 mg
  • Calcium Chloride....................................... 0.5 mg
  • Zinc Sulfate............................................... 1.0 mg
  • Copper Sulfate........................................... 1.0 mg
  • L-Glutamic Acid (sodium salt)..................... 0.5 g
  • Ferric Ammonium Citrate........................... 0.04 g
  • Pyridoxine Hydrochloride......................... 1.0 mg
  • Biotin......................................................... 0.5 mg
  • Malachite Green..................................... 250.0 μg
  • Agar............................................................ 15.0 g

Technical specifications

  • Dehydrated: Light beige to light beige with slight green tint, free-flowing, homogeneous
  • Reaction of 1.9%
  • pH 6.6 +/- 2 at 25°C
  • Prepared appearance: Light amber, slightly opalescent.

Packaging & Labelling

500 g in plastic bottle

Transport not regulated

Instructions for use

Storage

  • At room temperature below 30°C
  • Store in dry conditions and in a well-sealed receptacle, far from oxidizing substances.
  • Keep the container tightly closed.
  • Middlebrook 7H10 plates must be stored in the fridge and can be kept for up to 4 weeks as long as the plate does not get dry.

MSF requirements

Reserved for TB culture programmes: Used to prepare agar medium plates for Thin Layer Agar technique.

​It can also be used to prepare agar base tubes for Drug Susceptibility Testing on solid medium.

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