AURAMINE/RHODAMINE STAINING, 4 x 200 ml, kit
Valid Article
AURAMINE/RHODAMINE STAINING (Merck)
Feb 2021 - Merck informed MSF supply that this item is not for export/not available.
Is replaced by SLASAURA3K2 AURAMINE M FLUORESCENT STAIN KIT, TB (BD), 3 x 250 ml 212519
Definition
Fluorescent staining kit with auramine-rhodamine for the microscopic detection of mycobacteria.
Specifications
Liquid ready to use reagents:
- R1: auramine-rhodamine stain solution 1 x 200 ml
- R2: decolourization solution (C3H6O - HCl) 2 x 200 ml
- R3: counterstaining solution (KMNO4) 1 x 200 ml
Packaging & Labelling
4 bottles of 200 ml
Transport Dangerous Goods
- UN2924
- Class: 3 (8)
- Packing group: I
- Proper Shipping Name: Flammable liquid, corrosive, n.o.s.
Instructions for use
To be used with a fluorescent microscope (see related articles below).
Kit sufficient for 60-65 preparations.
In routine laboratory after good quality staining is established, switch to home made auramine with auramine O and potassium permanganate which is much cheaper than using this ready to use kit.
Storage
- Protected from humidity
- Between 15º and 25ºC
- Expiry date on the bottle
Classification EC Regulation N° 1272/2008
Auramine-rhodamine solution
- Flammable liquid: Category 3, H226
- Eye irritation: Category 2, H319
Decolourization solution
- Flammable liquid: Category 2, H225
- Eye irritation: Category 2, H319
- Specific target organ toxicity - single exposure: Category 3, H336
Counterstaining solution
- Chronic aquatic toxicity: Category 2, H411
Signal Word
Auramine-rhodamine solution: Warning
H226 | Flammable liquid and vapour. |
H319 | Causes serious eye irritation |
H336 | May cause drowsiness or dizziness |
P210 | Keep away from heat/sparks/open flames/hot surfaces. – No smoking. |
P305 + P351 + P338 | IF IN EYES: rinse cautiously with water for several minutes. If the victim wears contact lenses, remove them if possible. Keep rinsing |
Signal Word
Decolourization solution: Danger
H225 | Highly flammable liquid and vapour |
H319 | Causes serious eye irritation |
H336 | May cause drowsiness or dizziness |
P210 | Keep away from heat/sparks/open flames/hot surfaces. – No smoking. |
P305 + P351 + P338 | IF IN EYES: rinse cautiously with water for several minutes. If the victim wears contact lenses, remove them if possible. Keep rinsing |
Signal Word
Counterstaining solution: Danger
H411 | Toxic to aquatic life with long lasting effects. |
P273 | Avoid release to the environment |
MSF requirements
Staining kit necessary for the detection of mycobacteria with a fluorescence microscope.