GRAM HUCKER, bacterio. (RAL), 4 x 240 ml, kit
Valid Article
GRAM HUCKER, bacterio. (RAL), solution
Definition
Kit of 4 ready to use reagents for the Gram staining.
Specifications
Components
- Oxalate violet crystal contains CI Basic Violet 3
- Lugol's iodine contains Iodize potassium
- Differentiator (ethanol) contains butan-1-ol; n-butanol
Technical specifications
- Liquid ready to use reagents:
- R1: Oxalate violet crystal. Clear dark violet liquid
- R2: Lugol's iodine. Clear brown liquid
- R3: Differentiator (ethanol). Colourless liquid
- R4: Safranine. Clear red liquid
- Quality: brandname RAL
Packaging & Labelling
4 plastic bottles of 240 ml
Transport Dangerous Goods
Crystal Violet Oxalate, Differentiator and Safranine
- UN 1170
- Class: 3
- Packing group: III
- Proper shipping name: Ethanol solution
Instructions for use
The kit is sufficient for 230 to 300 slides provided a minimum reagent quantity (1 ml max) is put on the smear. If the slides are covered, the kit is sufficient for 100 determinations.
Do not stain too many slides simultaneously to ensure that sufficient time is allocated to the staining.
Tightly close the bottle after use to prevent evaporation or contamination with atmospheric humidity.
Please consult the “Updated laboratory procedures, 2022” or “Bacteriology laboratory procedures and resources“ available online via the Laboratory working Group sharepoint page: Laboratory Procedures and Resources.
https://msfintl.sharepoint.com/sites/msfintlcommunities/LabWG/SitePages/Home.aspx
For offline access, contact your laboratory advisor.
Storage
- Between 15ºC and 25ºC
- Keep away from light
- Expiry date on the bottle
- Shelf life: 18 months
- Guaranteed minimum remaining shelf life at delivery: 1/3 of total shelf life
Caution! Parcels with IATA packaging (R1, R3 et R4) are marked "ETHANOL", which can be confusing for proper identification of the article.
Waste management
Presence of substance with potenital carcinogenic properties (H350) and hazardous characteristics. Please contact your watsan referent for advice on proper disposal.
Detailed hazard and precautionary information can be found in the safety data sheet (SDS).
Classification EC Regulation N° 1272/2008
Crystal Violet oxalate:
- Flammable liquids, Category 3 H226
- Serious eye damage/eye irritation, Category 2 H319
- Carcinogenicity, Category 2 H351
- Hazardous to the aquatic environment – Chronic Hazard, Category3 H412
Lugol, PVP-stabilised solution:
- Specific target organ toxicity – Repeated exposure, Category 2 H373
Differenciator (alcohol-based):
- Flammable liquids, Category 2 H225
- Serious eye damage/eye irritation, Category 1 H318
Safranine:
- Flammable liquids, Category 3 H226
Signal Word
Crystal Violet Oxalate: Warning
H226 | Flammable liquid and vapour. |
H319 | Causes serious eye irritation |
H351 | Suspected of causing cancer (state route of exposure if it is conclusively proven that no other routs of exposure cause the hazard). |
H412 | Harmful to aquatic life with long lasting effects |
P210 | Keep away from heat/sparks/open flames/hot surfaces. – No smoking. |
P280 | Wear protective gloves/protective clothing/eye protection/face protection |
P308 + P313 | IF exposed or concerned: Get medical advice/attention. |
P337 + P313 | If eye irritation persists: get medical advice/attention |
Signal Word
Lugol: Warning
H373 | May cause damage to organs (or state all organs affected, if known) through prolonged or repeated exposure (state route of exposure if it is conclusively proven that no other routes of exposure cause the hazard). |
P314 | Get medical advice/attention if you feel unwell. |
Signal Word
Safranine: Warning
Differenciator: Danger