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GRAM HUCKER, bacterio. (RAL), 4 x 240 ml, kit

https://unicat.msf.org/cat/product/18789
STD SLASGRAM4--

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Shelf life = length of time a product can remain active and effective. Short shelf life = shelf life = < 24 months
W01030103 - Cellular stains for microscopy
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The order of this product needs to be justified and is only acceptable under certain conditions.
UN1170
Hazardous materials and products (identified by their 4-digit transport UN-number) must be transported under certain conditions as specified in the MSDS sheet (Material Safety Data Sheet).
1525
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GRAM HUCKER, bacterio. (RAL), solution

Definition

Kit of 4 ready to use reagents for the Gram staining.

Specifications

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Components

  • Oxalate violet crystal contains CI Basic Violet 3
  • Lugol's iodine contains Iodize potassium
  • Differentiator (ethanol) contains butan-1-ol; n-butanol

Technical specifications

  • Liquid ready to use reagents:
    • R1: Oxalate violet crystal. Clear dark violet liquid
    • R2: Lugol's iodine. Clear brown liquid
    • R3: Differentiator (ethanol). Colourless liquid
    • R4: Safranine. Clear red liquid
  • Quality: brandname RAL

Packaging & Labelling

4 plastic bottles of 240 ml

Transport Dangerous Goods

Crystal Violet Oxalate, Differentiator and Safranine

  • UN 1170
  • Class: 3
  • Packing group: III
  • Proper shipping name: Ethanol solution

Instructions for use

The kit is sufficient for 230 to 300 slides provided a minimum reagent quantity (1 ml max) is put on the smear. If the slides are covered, the kit is sufficient for 100 determinations.

Do not stain too many slides simultaneously to ensure that sufficient time is allocated to the staining.

Tightly close the bottle after use to prevent evaporation or contamination with atmospheric humidity.

Please consult the “Updated laboratory procedures, 2022” or “Bacteriology laboratory procedures and resources“ available online via the Laboratory working Group sharepoint page: Laboratory Procedures and Resources.

https://msfintl.sharepoint.com/sites/msfintlcommunities/LabWG/SitePages/Home.aspx

For offline access, contact your laboratory advisor.

Storage

  • Between 15ºC and 25ºC
  • Keep away from light
  • Expiry date on the bottle
  • Shelf life: 18 months
  • Guaranteed minimum remaining shelf life at delivery: 1/3 of total shelf life

Caution! Parcels with IATA packaging (R1, R3 et R4) are marked "ETHANOL", which can be confusing for proper identification of the article.

Waste management

Presence of substance with potenital carcinogenic properties (H350) and hazardous characteristics. Please contact your watsan referent for advice on proper disposal.

Detailed hazard and precautionary information can be found in the safety data sheet (SDS).

Classification EC Regulation N° 1272/2008

Crystal Violet oxalate:

  • Flammable liquids, Category 3 H226
  • Serious eye damage/eye irritation, Category 2 H319
  • Carcinogenicity, Category 2 H351
  • Hazardous to the aquatic environment – Chronic Hazard, Category3 H412

Lugol, PVP-stabilised solution:

  • Specific target organ toxicity – Repeated exposure, Category 2 H373

Differenciator (alcohol-based):

  • Flammable liquids, Category 2 H225
  • Serious eye damage/eye irritation, Category 1 H318

Safranine:

  • Flammable liquids, Category 3 H226

Signal Word

Crystal Violet Oxalate: Warning

Image Image Image
H226Flammable liquid and vapour.
H319Causes serious eye irritation
H351Suspected of causing cancer (state route of exposure if it is conclusively proven that no other routs of exposure cause the hazard).
H412Harmful to aquatic life with long lasting effects
P210Keep away from heat/sparks/open flames/hot surfaces. – No smoking.
P280Wear protective gloves/protective clothing/eye protection/face protection
P308 + P313IF exposed or concerned: Get medical advice/attention.
P337 + P313If eye irritation persists: get medical advice/attention

Signal Word

Lugol: Warning

Image
H373May cause damage to organs (or state all organs affected, if known) through prolonged or repeated exposure (state route of exposure if it is conclusively proven that no other routes of exposure cause the hazard).
P314Get medical advice/attention if you feel unwell.

Signal Word

Safranine: Warning

Differenciator: Danger

Image Image
H225Highly flammable liquid and vapour
H226Flammable liquid and vapour.
H318Causes serious eye damage.
P210Keep away from heat/sparks/open flames/hot surfaces. – No smoking.
P280Wear protective gloves/protective clothing/eye protection/face protection
P305 + P351 + P338 + P310IF IN EYES: Rinse cautiously with water for several minutes. Remove contact lenses, if present and easy to do. Continue rinsing. Immediately call a POISON CENTER/doctor

MSF requirements

Complete ready to use kit for the Gram staining.

Oxalate crystal violet does not need to be filtered.

Adding polyvinyl pyrrolidone to Lugol's solution allows it to be better preserved in a plastic bottle.

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