MIDDLEBROOK 7H11 AGAR, dehydrated, 500g


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Former Code(s): SLASMIDB11- SLASMIDB101 SLASZNL0168
W0104010101 - Dehydrated culture media (dcm)
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MIDDLEBROOK 7H11 Agar, dehydrated

Reserved for TB culture programmes.


Agar to prepare Middlebrook 7H11 media. Used with glycerol, Middlebrook OADC Enrichment and antibiotic / antimycotic mixture for isolation, cultivation and susceptibility testing of mycobacteria.


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Liofilchem typical formula:

  • Pancreatic Digest of Casein 1.0 g/L
  • L-Glutamic Acid 0.5 g/L
  • Sodium Citrate 0.4 g/L
  • Pyridoxine Hydrochloride 0.001 g/L
  • Biotin 0.0005 g/L
  • Ferric Ammonium Citrate 0.04 g/L
  • Ammonium Sulfate 0.5 g/L
  • Disodium Phosphate 1.5 g/L
  • Monopotassium Phosphate 1.5 g/L
  • Magnesium Sulfate 0.05 g/L
  • Malachite Green 0.001 g/L
  • Agar 15.0 g/L

Becton Dickinson and company formula (Approximate Formula per 900 mL):

  • ​Pancreatic Digest of Casein 1.0 g
  • Monosodium Glutamate 0.5 g
  • Sodium Citrate 0.4 g
  • Pyridoxine 1.0 mg
  • Biotin 0.5 mg
  • Ferric Ammonium Citrate 0.04 g
  • Ammonium Sulfate 0.5 g
  • Disodium Phosphate 1.5 g
  • Monopotassium Phosphate 1.5 g
  • Magnesium Sulfate 0.05 g
  • Agar 13.5 g
  • Malachite Green 0.25 mg
  • Zinc Sulfate 1.0 mg
  • Copper Sulfate 1.0 mg
  • Calcium Chloride 0.5 mg

Technical specifications

  • BD: dehydrated: Light beige powder; prepared apperance: colorless to pale tan to light tan-green, slightly hazy to hazy.
  • Liofilchem dehydrated: beige powder
  • Main components: salts, oleic acid, albumine, catalase, caseine hydrolisate
  • ​Soluble in water

Packaging & Labelling

  • 500 g in plastic bottle
  • Transport not regulated

Instructions for use

Dissolve Middlebrook 7H11 in distilled water and add glycerol according to standard procedures.

Autoclave before adding antibiotic/antimycotic mixture.

When preparing Middlebrook 7H11 enriched with 4- Nitrobenzoic acid, add the acid solution before autoclaving the mixture.

While pouring plates, keep Middlebrook 7H11 water bath at 50°C, as agar solidifies very quickly.

Perform regular medium Quality Control for each new batch of plates prepared.

Middlebrook 7H11 agar should incubate at a 5% CO2 atmosphere.


  • At room temperature between 10 and 30°C
  • Store in dry conditions and in a well-sealed receptacle, far from oxidizing substances.
  • Middlebrook 7H11 plates must be stored in the fridge and can be kept for up to 4 weeks as long as the plate does not get dry.

MSF requirements

Reserved for TB culture programmes: Used to prepare agar medium plates for Thin Layer Agar technique.

It can also be used to prepare agar base tubes for Drug Susceptibility Testing on solid medium.

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