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MIDDLEBROOK 7H11 AGAR, dehydrated, 500g

https://unicat.msf.org/cat/product/18816
STD SBCMAGAR7H11

Valid Article

Former Code(s): SLASMIDB11- SLASMIDB101 SLASZNL0168
W0104010101 - Dehydrated culture media (dcm)
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CT30
Thermosensitive codes are defined for storage and transportation temperature requirements of the products.
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MIDDLEBROOK 7H11 Agar, dehydrated

Reserved for TB culture programmes.

Definition

Agar to prepare Middlebrook 7H11 media. Used with glycerol, Middlebrook OADC Enrichment and antibiotic / antimycotic mixture for isolation, cultivation and susceptibility testing of mycobacteria.

Specifications

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Components

Liofilchem typical formula:

  • Pancreatic Digest of Casein 1.0 g/L
  • L-Glutamic Acid 0.5 g/L
  • Sodium Citrate 0.4 g/L
  • Pyridoxine Hydrochloride 0.001 g/L
  • Biotin 0.0005 g/L
  • Ferric Ammonium Citrate 0.04 g/L
  • Ammonium Sulfate 0.5 g/L
  • Disodium Phosphate 1.5 g/L
  • Monopotassium Phosphate 1.5 g/L
  • Magnesium Sulfate 0.05 g/L
  • Malachite Green 0.001 g/L
  • Agar 15.0 g/L

Becton Dickinson and company formula (Approximate Formula per 900 mL):

  • ​Pancreatic Digest of Casein 1.0 g
  • Monosodium Glutamate 0.5 g
  • Sodium Citrate 0.4 g
  • Pyridoxine 1.0 mg
  • Biotin 0.5 mg
  • Ferric Ammonium Citrate 0.04 g
  • Ammonium Sulfate 0.5 g
  • Disodium Phosphate 1.5 g
  • Monopotassium Phosphate 1.5 g
  • Magnesium Sulfate 0.05 g
  • Agar 13.5 g
  • Malachite Green 0.25 mg
  • Zinc Sulfate 1.0 mg
  • Copper Sulfate 1.0 mg
  • Calcium Chloride 0.5 mg

Technical specifications

  • Light beige powder
  • Main components: salts, oleic acid, albumine, catalase, caseine hydrolisate
  • ​Soluble in water

Packaging & Labelling

  • 500 g in plastic bottle
  • Transport not regulated

Instructions for use

Dissolve Middlebrook 7H11 in distilled water and add glycerol according to standard procedures.

Autoclave before adding antibiotic/antimycotic mixture.

When preparing Middlebrook 7H11 enriched with 4- Nitrobenzoic acid, add the acid solution before autoclaving the mixture.

While pouring plates, keep Middlebrook 7H11 water bath at 50°C, as agar solidifies very quickly.

Perform regular medium Quality Control for each new batch of plates prepared.

Middlebrook 7H11 agar should incubate at a 5% CO2 atmosphere.

Storage

  • At room temperature between 10 and 30°C
  • Store in dry conditions and in a well-sealed receptacle, far from oxidizing substances.
  • Middlebrook 7H11 plates must be stored in the fridge and can be kept for up to 4 weeks as long as the plate does not get dry.

MSF requirements

Used to prepare agar medium plates for Thin Layer Agar technique.

It can also be used to prepare agar base tubes for Drug Susceptibility Testing on solid medium.

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