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  3. (rapid smear stain,RAL555) METHYLENE BLUE,REFILL R3,1 l,bot.
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(rapid smear stain,RAL555) METHYLENE BLUE,REFILL R3,1 l,bot.

https://unicat.msf.org/cat/product/18843
STD SLASRALM1B1

Valid Article

Former Code(s): -X
1525
Thermosensitive codes are defined for storage and transportation temperature requirements of the products.
OC subscriptions: included in supply or field order tools (e.g. UniField)
SC subscriptions: included in supply or field tools
OC validations: approved for procurement and use by an OC for international or local orders specifying context and activity in Medical Standard Lists (MSL)

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RAPID SMEARS STAINING (RAL 555) sol.

Definition

Kit of 3 ready to use reagents for the staining of blood smears.

Specifications

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Components

  • Eosin-RAL 555 is composed of a mixture of: 5-chloro-2-methyl-2H-isothiazol-3-one [EC No 247-500-7] and 2-
    methyl-2H-isothiazol-3-one [EC No 220-239-6] (3:1)

Technical specifications

  • CAS n°: 61-73-4
  • Composition:
    • R1: fixative 100 ml = methanol (FIX-RAL 555)
    • R2: 0.08 g eosin + pH 7 buffer water 100 ml (EOSIN-RAL 555)
    • R3: 0.12 g methylene blue + pH 7 buffer water 100 ml (BLUE-RAL 555)
  • Liquid ready to use reagents
  • Quality: brandname RAL
  • FIX-RAL 555: Clear violet liquid
  • BLUE-RAL 555: Clear dark blue liquid
  • EOSIN-RAL 555: Red/orange clear liquid

Packaging & Labelling

3 plastic bottles of 100 ml

Transport Dangerous Goods

FIX-RAL 555 :

  • UN1230
  • Class: 3 (6.1)
  • Packing group: II
  • Proper shipping name: Methanol

Instructions for use

The kit is sufficient for 100 to 200 slides if the manufacturer's procedure is followed.

First choice for the staining of blood smears.

Although the kit allows to detect parasites, the kit is not to be used for thick blood films since methanol fixes red blood cells (use Giemsa).

Tightly close the bottles after use to prevent evaporation or contamination with atmospheric humidity. Beware, methanol is particularly hygroscopic.

Follow exactly the staining times.

Allow the slide to drip dry between the R2 and R3 solution.

Can not be used to do a series of slides.

R1, R2 and R3 can be ordered separately.

Please consult the “Updated laboratory procedures, 2022” available online via the Laboratory working Group sharepoint page: Laboratory Procedures and Resources.

https://msfintl.sharepoint.com/sites/msfintlcommunities/LabWG/SitePages/Laboratory-Manual-page.aspx

For offline access, contact your laboratory advisor.

Storage

  • At room temperature
  • Store FIX-RAL and BLUE-RAL between 15 and 25°C
  • R2 and R3 solutions have limited shelf life and should be used within two months after opening

CAUTION:

Parcels with IATA packaging are marked "METHANOL", which can result in errors when storing the goods.

Waste management

Substance with hazardous characteristics. Please contact your watsan referent for advice on proper disposal.

Detailed hazard and precautionary information can be found in the safety data sheet (SDS).

Classification EC Regulation N° 1272/2008

FIX-RAL 555

  • Flammable liquids, Category 2 H225
  • Acute toxicity (oral), Category 3 H301
  • Acute toxicity (dermal), Category 3 H311
  • Acute toxicity (inhalation:vapour) Category 3 H331
  • Specific target organ toxicity — single exposure, Category 1 H370

EOSIN-RAL 555

  • Skin sensitisation, Category 1 H317
  • Hazardous to the aquatic environment — Chronic Hazard, Category 3 H412

Signal Word

FIX-RAL 555: Danger

Image Image Image
H225Highly flammable liquid and vapour
H370Causes damage to organs (or state all organs affected, if known) (state route of exposure if it is conclusively proven that no other routes of exposure cause the hazard).
H301 + H311 + H331Toxic if swallowed, in contact with skin or if inhaled
P210Keep away from heat/sparks/open flames/hot surfaces. – No smoking.
P261Avoid breathing dust/fume/gas/mist/vapours/spray.
P264Wash … thoroughly after handling
P280Wear protective gloves/protective clothing/eye protection/face protection
P301 + P310IF SWALLOWED: Immediately call a POISON CENTER or doctor/physician.
P308 + P311IF exposed or concerned: Call a POISON CENTER or doctor.

Signal Word

EOSIN-RAL 555 : Warning

Image
H317May cause an allergic skin reaction
H412Harmful to aquatic life with long lasting effects
P261Avoid breathing dust/fume/gas/mist/vapours/spray.
P280Wear protective gloves/protective clothing/eye protection/face protection
P333 + P313If skin irritation or rash occurs: Get medical advice/attention.
P362 + P364Take off contaminated clothing and wash it before reuse.

MSF requirements

Rapid staining (15 to 20 seconds per slide).

Good differentiation of blood cells and parasites in blood smears.

Refills of reagents readily available, interesting volumes for laboratories with important activity.

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