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TRYPTIC SOY BLOOD AGAR BASE n°2, dehydrated, 500g

https://unicat.msf.org/cat/product/18874
NST SBCMAGARTSBA

Discontinued Article

Former Code(s): SLASTSBA2--
P
The order of this product needs to be justified and is only acceptable under certain conditions.
CT30
Thermosensitive codes are defined for storage and transportation temperature requirements of the products.
OC subscriptions: included in supply or field order tools (e.g. UniField)
SC subscriptions: included in supply or field tools
OC validations: approved for procurement and use by an OC for international or local orders specifying context and activity in Medical Standard Lists (MSL)

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TRYPTIC SOY BLOOD AGAR BASE N°2

2021: Stopped because no longer available from BD and no longer used in microbiology

Definition

Agar base is enriched with blood for the in-house preparation of blood agar. It is an enriched medium extensively used for cultivation and isolation of microorganisms.

Synonym

Soybean Casein digest Agar, TSA, CASO Agar, Tryptone Soya Agar.

Specifications

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Technical specifications

  • Light beige homogenous powder
  • Composition: Tryptone H, Soytone, Sodium Chloride, Agar
  • Soluble in water

Packaging & Labelling

  • 500 g powder in plastic bottle
  • Transport not regulated

Instructions for use

  • ​Follow Standard Operational Procedures for Blood Agar plates preparation.
  • Ensure aseptic conditions during medium preparation.
  • Once prepared store Blood Agar plates refrigerated 2-8ºC. Use no longer than 4 weeks after preparation.
  • ​Before use, perform internal quality control. To check for sterility, incubate few plates from each new batch for 2 days at 36ºC.
  • Add defibrinated blood when agar has cooled down to avoid haemolysis.

Storage

  • At room temperature below 30°C
  • Store in dry conditions in well sealed receptacles far from oxidizing substances
  • Shelf life: 6 months

(Cf Introduction: Thermosensitive products)

MSF requirements

Agar Base is used for in-house preparation of Blood Agar plates. Those are used to check if broth from positive MGIT cultures contains contaminants.

It is recommended to purchase blood agar plates already prepared if in-house preparation of blood agar plates is not a routine laboratory procedure. Refer to your laboratory advisor.

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