MIDDLEBROOK 7H9 BROTH, dehydrated [BD-271310]

STD SBCMBRTH7H9D

Valid Article

W0104010101 - Dehydrated culture media (dcm)
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MIDDLEBROOK 7H9 Broth, dehydrated

Reserved for TB culture programmes.

Definition

Broth medium prepared from powder. It is used for routine diagnosis and suspension preparation of Mycobacterium tuberculosis.

Synonym

Middlebrook 7H9 broth

Specifications

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Components

Approximative formula per 900 ml

  • Ammonium Sulfate..................................................... 0.5 g
  • L-Glutamic Acid........................................................... 0.5 g
  • Sodium Citrate............................................................. 0.1 g
  • Pyridoxine.................................................................... 1.0 mg
  • Biotin........................................................................... 0.5 mg
  • Disodium Phosphate.................................................... 2.5 g
  • Monopotassium Phosphate.......................................... 1.0 g
  • Ferric Ammonium Citrate............................................. 0.04 g
  • Magnesium Sulfate...................................................... 0.05 g
  • Calcium Chloride......................................................... 0.5 mg
  • Zinc Sulfate.................................................................. 1.0 mg
  • Copper Sulfate............................................................. 1.0 mg

Technical specifications

  • Solid, powder, dehydrated
  • Light beige, free-flowing, homogeneous
  • Prepared appearance: colorless to very light amber, clear
  • Characteristic odour

Packaging & Labelling

Botlle of 500g

Instructions for use

Storage

  • Keep below 30°C
  • Do not freeze or overheat
  • Store away from oxidizing substances
  • Open just before use

(Cf Introduction: Thermosensitive products)

MSF requirements

Reserved for TB culture programmes: It is used to prepare mycobacterial suspensions, required for Quality Control of new batches of BBL MGIT tubes or to perform Drug Susceptibility Test of isolates from solid media.

​Middlebrook 7H9 with glycerol is also used as a preservation medium for the storage of isolates at <-18ºC.

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