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HANDLE for Pasteur loop

https://unicat.msf.org/cat/product/13110
STD ELABMILP1--

Valid Article

Former Code(s): ELAEMILP1--
W05030380 - Microbiology devices - accessories
European Medical Device Nomenclature (EMDN) is the nomenclature of use by manufacturers when registering their medical devices in the EUDAMED database. EMDN is characterised by its alphanumeric structure that is established in a seven-level hierarchical tree.
The product is part of at least one Kit. A kit is a collection of products (medical and/or logistic) that are needed for a certain intervention in emergency. The choice and quantity of the articles reflects the MSF protocols for this specific situation. The use of Kits allows to start an intervention without a detailed evaluation.
OC subscriptions: included in supply or field order tools (e.g. UniField)
SC subscriptions: included in supply or field tools
OC validations: approved for procurement and use by an OC for international or local orders specifying context and activity in Medical Standard Lists (MSL)

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PASTEUR HANDLE, LOOP and INOCULATION NEEDLE

Definition

An inoculation loop is a simple tool used to gather and transfer microscopic organisms from a culture or broth plate. The small loop removes a consistent amount of inoculum by using surface tension.

An inoculation needle is used for transfer and repicking of bacterial colonies and for the inoculation of Kligler Hajna media.

Both are used with an Pasteur handle.

Synonym

Microloop Pasteur, Wire loop, Culture or Culture media inoculating, streaker.

Specifications

Technical specifications

HANDLE

  • Stainless steel or aluminium
  • Length: ± 285 mm
  • Hollow end (0.3 - 0.6 mm) to insert the wire of the loop or the inoculation needle

LOOP

  • Nickel-chromium
  • Length of the wire: > 45 mm
  • Ø of the wire: 0.6 mm
  • Ø of the loop: +/- 5 mm
  • Volume: 10µl

INOCULATION NEEDLE

  • Stainless steel
  • Length of the wire: > 35 mm

Instructions for use

The inoculation loop and inoculation needle are flame-sterilized before and after each use to eliminate cross-contamination between cultures. The loop and needle must be cooled after sterilization to avoid destroying the organisms.

In TB laboratory, the loop is used to take a sample of sputum and spread it on a slide for detecting the Koch's bacillus.

Possible alternative: single use plastic loop (see related articles below).

MSF requirements

Some tests in bacteriology laboratories, require the use of a calibrated loop with a specific volume. This is not the case for all tests, for example smears on slides.

Avoid wire from a reel (loop more difficult to make - no precise volume).

Alternative: loop fastened to the handle, platinum model.

Some suppliers sell handles and loops/needles separately. Check for compatibility.

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