(spectro) KIT ADENOSINE DEAMINASE 1x50ml + 1x25ml (Diazyme)
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(spectrophotometer) TEST ADA (Diazyme)
Definition
Biochemical, enzymatic test for the determination of the acitivity of adenosine déaminase in peritoneal or pleural fluids.
Test used to help for the diagnosis of M. tuberculosis.
Specifications
Standard kit of 250 tests.
Components
- Reagent R1 = 1x 50 ml (ref DZ117A-R1)
- Tris HCl, pH 8.0 50 mM
- 4-AA 2 mM
- PNP 0.1 U/ml
- XOD 0.2 U/ml
- Peroxidase 0.6 U/ml
- Stabilizers
- Reagent R2 = 1x 25 ml (ref DZ117A-R2)
- Tris-HCl, pH 4.0 50 mM
- Adenosine 10 mM
- EHSPT 2 mM
The reagent kit is supplied with Adenosine Deaminase Calibrator = 1x1ml vial, lyophilized
Technical specifications
Kit for 250 measurement (controls, calibrator, samples)
To be Ordered Separately
- Calibrator (ref DZ117A-CAL) - 1 vial included in the test kit
- 2 controls (ref DZ117A-CON)
See related article below
Instructions for use
Use on pleural liquid samples.
Use the calibrator, the high and the low control.
Please consult the “Updated laboratory procedures, 2022” available online via the Laboratory working Group sharepoint page: Laboratory Procedures and Resources.
https://msfintl.sharepoint.com/sites/msfintlcommunities/LabWG/SitePages/Laboratory-Manual-page.aspx
For offline access, contact your laboratory advisor.
Precautions for Use
All biological samples must be treated as potentially infectious and handled with the usual precautions (compulsary wearing of gloves, hand washing, etc.).
Storage
- Keep refrigerated between 2 and 8ºC
- Do not freeze
- Shelf life: 12 months
- Guaranteed minimum remaining shelf life at delivery: 1/3 of total shelf life
- Not to be used beyond expiry date
- R1 is light-sensitive. Store in a dark place.
MSF requirements
ADA is a biomarker for the diagnosis of pleural TB.