METHYLENE BLUE, powder, 100 g, bot.


Valid Article

W01030103 - Cellular stains for microscopy
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METHYLENE BLUE, Löffler's solution


Powder reagent used to prepare the aqueous solution of methylene blue or ready to use aqueous solution for microscopy and cell counting.


Methylthionine chloride, Löffler's/Loeffler methylene blue solution


Technical specifications

CAS N°: 61-73-4


  • Formula: C16H18ClN3S
  • Quality: for microscopy or bacteriology


  • Composition of methylene Blue solution according to Löffler's/Loeffler:
    • methylene blue 0.3 g
    • ethanol 30 ml
    • water 100 ml
  • Formula: C16H18CIN3S . 3H2O
  • Quality: for microscopy or bacteriology

Packaging & Labelling

100g, 100 ml or 500 ml glass or plastic bottle

Transport Dangerous Goods

Solution only: regulated transport according some manufacturers. To be checked!

  • No UN code or
  • UN1993
    • Class: 3
    • Packing group: III
    • Proper shipping name: Flammable liquid, toxic, n.o.s. (CONT. ETHANOL AND ETHYL METHYL KETONE)

Instructions for use

  • Used for Ziehl-Neelsen staining
  • Used for the preparation of the Türk solution
  • For stainings: use a 3% solution to detect the morphology of bacteria, especially meningococci in cerebrospinal fluid (CSF) and gonococci in uraemic specimens.

Please consult the “Updated laboratory procedures, 2022” or “Bacteriology laboratory procedures and resources“ available online via the Laboratory working Group sharepoint page: Laboratory Procedures and Resources.

For offline access, contact your laboratory advisor.


  • At room temperature
  • Protected from humidity
  • Expiry date on the bottle

Waste management

Substance with hazardous characteristics. Please contact your watsan referent for advice on proper disposal.

Detailed hazard and precautionary information can be found in the safety data sheet (SDS).

Classification EC Regulation N° 1272/2008


  • Acute toxicity, Oral: Category 4, H302


  • Flammable liquid: Category 3, H226

Signal Word

POWDER: Warning

H302Harmful if swallowed
P301 + P312IF SWALLOWED: call a POISON CENTER or doctor/physician if you feel

Signal Word


H226Flammable liquid and vapour.
P301 + P312IF SWALLOWED: call a POISON CENTER or doctor/physician if you feel
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