METHYLENE BLUE, Löffler’s solution, 500 ml, bot.
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METHYLENE BLUE, Löffler's solution
Definition
Powder reagent used to prepare the aqueous solution of methylene blue or ready to use aqueous solution for microscopy and cell counting.
Synonym
Methylthionine chloride, Löffler's/Loeffler methylene blue solution
Specifications
Technical specifications
CAS N°: 61-73-4
Powder
- Formula: C16H18ClN3S
- Quality: for microscopy or bacteriology
Solution
- Composition of methylene Blue solution according to Löffler's/Loeffler:
- methylene blue 0.3 g
- ethanol 30 ml
- water 100 ml
- Formula: C16H18CIN3S . 3H2O
- Quality: for microscopy or bacteriology
Packaging & Labelling
100g, 100 ml or 500 ml glass or plastic bottle
Transport Dangerous Goods
Solution only: regulated transport according some manufacturers. To be checked!
- No UN code or
- UN1993
- Class: 3
- Packing group: III
- Proper shipping name: Flammable liquid, toxic, n.o.s. (CONT. ETHANOL AND ETHYL METHYL KETONE)
Instructions for use
- Used for Ziehl-Neelsen staining
- Used for the preparation of the Türk solution
- For stainings: use a 3% solution to detect the morphology of bacteria, especially meningococci in cerebrospinal fluid (CSF) and gonococci in uraemic specimens.
Please consult the “Updated laboratory procedures, 2022” or “Bacteriology laboratory procedures and resources“ available online via the Laboratory working Group sharepoint page: Laboratory Procedures and Resources.
https://msfintl.sharepoint.com/sites/msfintlcommunities/LabWG/SitePages/Home.aspx
For offline access, contact your laboratory advisor.
Storage
- At room temperature
- Protected from humidity
- Expiry date on the bottle
Waste management
Substance with hazardous characteristics. Please contact your watsan referent for advice on proper disposal.
Detailed hazard and precautionary information can be found in the safety data sheet (SDS).
Classification EC Regulation N° 1272/2008
POWDER
- Acute toxicity, Oral: Category 4, H302
SOLUTION
- Flammable liquid: Category 3, H226
Signal Word
POWDER: Warning
H302 | Harmful if swallowed |
P301 + P312 | IF SWALLOWED: call a POISON CENTER or doctor/physician if you feel |
Signal Word
SOLUTION: Warning
H226 | Flammable liquid and vapour. |
P301 + P312 | IF SWALLOWED: call a POISON CENTER or doctor/physician if you feel |