(APISyst) NIT1-NIT2 reagents (10S,20E,20NE,STAPH)[BMX-70442]
Valid Article
NIT1 & NIT2, API reagents (20E, 10S, 20NE, Staph, Coryne)
Definition
NIT1 and NIT 2 reagents are to be used with API strips, for the identification of enterobacteriae and other Gram negatif bacilli non fastidious (API 20 E), Gram negative bacilli, non-enterobacteriae and non fastidious (API 20 NE) or staphylococcus (API STAPH) (and other strips: Coryne and 10S).
Synonym
NIT 1: sulfanic acid
NIT 2: NN dimethyl-alpha-naphtylamine
Specifications
Components
NIT 1: Sulfanilic acid, acetic acid and H2O
NIT 2: N,N-dimethyl-1-naphtylamine, acetic acid and H2O
Technical specifications
NIT 1 and NIT 2: clear liquids, nearly colourless
Packaging & Labelling
Kit composed of 2 reagents: 2 x NIT 1 and 2 x NIT 2
4 x 5 ml
Transport Dangerous Goods
NIT 1 and NIT 2:
- UN 2790
- Class: 8
- Packing group: III
- Proper shipping name: Acetic acid solution, with more than 10% and less than 50% acid, by mass
Instructions for use
- Follow the instructions of the package insert
- Allow the reagents to come to room temperature before use
- Make sure that the reagents are put back in the refrigerator immediately after use
Please consult the “Bacteriology laboratory procedures and resources” available online via the Laboratory working Group sharepoint page: Laboratory Procedures and Resourceshttps://msfintl.sharepoint.com/sites/msfintlcommunities/LabWG/SitePages/Bacteriology-Laboratory-Resources.aspx
For offline access, contact your laboratory advisor
Precautions for Use
All biological samples must be considered as potentially infectious and handled with the usual precautions (compulsory wearing of gloves, hand washing, etc.).
Storage
- Store NIT 2 between 2°C and 8°C and NIT 1 beteen 2 and 30°C
- Keep away from light
- Do not use after the expiry date
- After opening, to be used within 1 month
- Shelf life: 11 months
- Guaranteed minimum remaining shelf life at delivery: 1/3 of the total shelf life
Waste management
After use, sterilize the cultures and all contaminated material.
Substance with hazardous characteristics. Please contact your watsan referent for advice on proper disposal.
Detailed hazard and precautionary information can be found in the safety data sheet (SDS).
Classification EC Regulation N° 1272/2008
NIT 1 and NIT 2:
- Skin corrosion/irritation, Category 1, H314
- Serious eye damage/eye irritation, Category 1
- Hazardous to the aquatic environment, long-term aquatic hazard, Category 3
Signal Word
Danger
H314 | Causes severe skin burns and eye damage. |
P264 | Wash … thoroughly after handling |
P280 | Wear protective gloves/protective clothing/eye protection/face protection |
P305 + P351 + P338 | IF IN EYES: rinse cautiously with water for several minutes. If the victim wears contact lenses, remove them if possible. Keep rinsing |
P337 + P313 | If eye irritation persists: get medical advice/attention |
Signal Word
Danger
H314 | Causes severe skin burns and eye damage. |
P264 | Wash … thoroughly after handling |
P273 | Avoid release to the environment |
P280 | Wear protective gloves/protective clothing/eye protection/face protection |
P305 + P351 + P338 | IF IN EYES: rinse cautiously with water for several minutes. If the victim wears contact lenses, remove them if possible. Keep rinsing |
P337 + P313 | If eye irritation persists: get medical advice/attention |
P501 | Dispose of contents/container to location in accordance with local and regional/ national/ international regulations |
MSF requirements
Reserved for bacteriology programmes