(API System) VP1-VP2 reagents (20E,STREP,STAPH) [BMX-70422]
Valid Article
VP1 & VP2, API reagents (20E, Staph, Strep)
Definition
VP1 and VP2 reagents are to be used with API strips, for the identification of enterobacteriae and other Gram negatif bacilli non fastidious (API 20 E), staphylococci (API STAPH) (or other strip: API Strep).
Synonym
VP1: potassium hydroxide
VP2: Alpha-naphtol
Specifications
Components
VP1: Potassium Hydroxyde and H2O, caustic potash
VP2: 1-naphtol, ethanol, ethyl alcohol
Technical specifications
VP1 and VP2 : clear liquid, (nearly) colourless
Packaging & Labelling
Kit composed of 2 reagents: 2 x VP1 and 2 x VP2
4 x 5 ml
Transport Dangerous Goods
VP1:
- UN 1814
- Class: 8
- Packing group: II
- Proper shipping name: Potassium hydroxide, solution
VP2:
- UN 1170
- Class: 3
- Packing group: II
- Proper shipping name: Ethanol or Ethyl alcohol or Ethanol solutions or Ethyl alcohol solutions
Instructions for use
- Follow the instructions of the package insert
- Allow the reagents to come to room temperature before use
- Make sure that the reagents are put back in the refrigerator immediately after use
Please consult the “Bacteriology laboratory procedures and resources” available online via the Laboratory working Group sharepoint page: Laboratory Procedures and Resources https://msfintl.sharepoint.com/sites/msfintlcommunities/LabWG/SitePages/Bacteriology-Laboratory-Resources.aspx
For offline access, contact your laboratory advisor
Precautions for Use
All biological samples must be considered as potentially infectious and handled with the usual precautions (compulsory wearing of gloves, hand washing, etc.).
Storage
- VP2 is stored between 2°C and 8°C and VP1, between 2°C and 30°C
- Keep away from light. Wrap the bottle of VP2 in aluminium foil after transfer
- Do not use after the expiry date
- After opening, to be used within the month
- Shelf life: 8 months
- Guaranteed minimum remaining shelf life at delivery: 1/3 of the total shelf life
Waste management
After use, sterilize the cultures and all contaminated material.
Substance with hazardous characteristics. Please contact your watsan referent for advice on proper disposal.
Detailed hazard and precautionary information can be found in the safety data sheet (SDS).
Classification EC Regulation N° 1272/2008
VP1:
- Acute toxicity, oral, Category 4, H302
- Skin corrosion/irritation, Category 1, H314
- Serious eye damage/eye irritation, Category 1
- Hazardous to the aquatic environment, long-term aquatic hazard, Category 3
VP2
- Flammable liquids, Category 2, H225
- Serious eye damage/eye irritation, Category 1, H318
- Hazardous to the aquatic environment, long-term aquatic hazard, Category 3, H412
Signal Word
Danger (VP1)
H302 | Harmful if swallowed |
H314 | Causes severe skin burns and eye damage. |
P403 | Store in a well-ventilated place. |
P280 | Wear protective gloves/protective clothing/eye protection/face protection |
P302 + P352 | IF ON SKIN: wash with plenty of soap and water |
P305 + P351 + P338 | IF IN EYES: rinse cautiously with water for several minutes. If the victim wears contact lenses, remove them if possible. Keep rinsing |
P337 + P313 | If eye irritation persists: get medical advice/attention |
Signal Word
Danger (VP2)
H225 | Highly flammable liquid and vapour |
H312 | Harmful in contact with skin. |
H318 | Causes serious eye damage. |
H412 | Harmful to aquatic life with long lasting effects |
P403 | Store in a well-ventilated place. |
P210 | Keep away from heat/sparks/open flames/hot surfaces. – No smoking. |
P280 | Wear protective gloves/protective clothing/eye protection/face protection |
P305 + P351 + P338 | IF IN EYES: rinse cautiously with water for several minutes. If the victim wears contact lenses, remove them if possible. Keep rinsing |
MSF requirements
Reserved for bacteriology programmes