(API System) VP1-VP2 reagents (20E,STREP,STAPH) [BMX-70422]

STD SBIDAPISVP-

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Former Code(s): SLASZBD0107
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W0104080208 - Single tests (discs, strips, droppers...)
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The order of this product needs to be justified and is only acceptable under certain conditions.
Hazardous materials and products (identified by their 4-digit transport UN-number) must be transported under certain conditions as specified in the MSDS sheet (Material Safety Data Sheet).
Thermosensitive codes are defined for storage and transportation temperature requirements of the products.
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VP1 & VP2, API reagents (20E, Staph, Strep)

Definition

VP1 and VP2 reagents are to be used with API strips, for the identification of enterobacteriae and other Gram negatif bacilli non fastidious (API 20 E), staphylococci (API STAPH) (or other strip: API Strep).

Synonym

VP1: potassium hydroxide

VP2: Alpha-naphtol

Specifications

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Components

VP1: Potassium Hydroxyde and H2O, caustic potash

VP2: 1-naphtol, ethanol, ethyl alcohol

Technical specifications

VP1 and VP2 : clear liquid, (nearly) colourless

Packaging & Labelling

Kit composed of 2 reagents: 2 x VP1 and 2 x VP2

4 x 5 ml

Transport Dangerous Goods

VP1:

  • UN 1814
  • Class: 8
  • Packing group: II
  • Proper shipping name: Potassium hydroxide, solution

VP2:

  • UN 1170
  • Class: 3
  • Packing group: II
  • Proper shipping name: Ethanol or Ethyl alcohol or Ethanol solutions or Ethyl alcohol solutions

Instructions for use

  • Follow the instructions of the package insert
  • Allow the reagents to come to room temperature before use
  • Make sure that the reagents are put back in the refrigerator immediately after use

Please consult the “Bacteriology laboratory procedures and resources” available online via the Laboratory working Group sharepoint page: Laboratory Procedures and Resources https://msfintl.sharepoint.com/sites/msfintlcommunities/LabWG/SitePages/Bacteriology-Laboratory-Resources.aspx

For offline access, contact your laboratory advisor

Precautions for Use

All biological samples must be considered as potentially infectious and handled with the usual precautions (compulsory wearing of gloves, hand washing, etc.).

Storage

  • VP2 is stored between 2°C and 8°C and VP1, between 2°C and 30°C
  • Keep away from light. Wrap the bottle of VP2 in aluminium foil after transfer
  • Do not use after the expiry date
  • After opening, to be used within the month
  • Shelf life: 8 months
  • Guaranteed minimum remaining shelf life at delivery: 1/3 of the total shelf life

(Cf Introduction: Thermosensitive products)

Waste management

After use, sterilize the cultures and all contaminated material.

Substance with hazardous characteristics. Please contact your watsan referent for advice on proper disposal.

Detailed hazard and precautionary information can be found in the safety data sheet (SDS).

Classification EC Regulation N° 1272/2008

VP1:

  • Acute toxicity, oral, Category 4, H302
  • Skin corrosion/irritation, Category 1, H314
  • Serious eye damage/eye irritation, Category 1
  • Hazardous to the aquatic environment, long-term aquatic hazard, Category 3

VP2

  • Flammable liquids, Category 2, H225
  • Serious eye damage/eye irritation, Category 1, H318
  • Hazardous to the aquatic environment, long-term aquatic hazard, Category 3, H412
Image Image

Signal Word

Danger (VP1)

H302Harmful if swallowed
H314Causes severe skin burns and eye damage.
P403Store in a well-ventilated place.
P280Wear protective gloves/protective clothing/eye protection/face protection
P302 + P352IF ON SKIN: wash with plenty of soap and water
P305 + P351 + P338IF IN EYES: rinse cautiously with water for several minutes. If the victim wears contact lenses, remove them if possible. Keep rinsing
P337 + P313If eye irritation persists: get medical advice/attention

Signal Word

Danger (VP2)

Image Image Image
H225Highly flammable liquid and vapour
H312Harmful in contact with skin.
H318Causes serious eye damage.
H412Harmful to aquatic life with long lasting effects
P403Store in a well-ventilated place.
P210Keep away from heat/sparks/open flames/hot surfaces. – No smoking.
P280Wear protective gloves/protective clothing/eye protection/face protection
P305 + P351 + P338IF IN EYES: rinse cautiously with water for several minutes. If the victim wears contact lenses, remove them if possible. Keep rinsing

MSF requirements

Reserved for bacteriology programmes

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