(API System) JAMES reagent (NH,10S,20E,20NE),kit [BMX-70542]
Valid Article
JAMES, reagent for API (NH, 20E, 20NE, 10S)
Definition
James reagent is to be used with API strips, for the identification of enterobacteriae and other Gram negatif bacilli non fastidious (API 20 E), Gram negative bacilli, non-enterobacteriae and non fastidious (API 20 NE), Neisseria or Haemophilus (API NH-included in kit) (and other strip: 10S).
Specifications
Components
R1: Hydrochloric acid 1N
R2: Compound J 2183 (confidential)
Technical specifications
R1: clear liquid, colourless or nearly colourless
R2: powder, solid, off-white before reconstitution and yellow after reconstitution
Packaging & Labelling
Kit composed of 2 reagents: R1 and R2
2 x 5 ml of R1 and 2 x 0.033 g of R2
Transport Dangerous Goods
R1:
- UN 1789
- Class: 8
- Packing group: III
- Proper shipping name: Hydrochloric acid
Instructions for use
- Follow the instructions of the package insert
- Allow the reagent to come to room temperature before use
- Make sure that the reagent is put back in the refrigerator immediately after use
- Wrap the dropper-bottle in aluminium foil to protect it from light
- The James reagent must be used only if it is pale yellow. If a pink color appears when the reagent is reconstituted with the solvent, wait until this pink color has completely disappeared before using it.
Please consult the “Bacteriology laboratory procedures and resources” available online via the Laboratory working Group sharepoint page: Laboratory Procedures and Resources
For offline access, contact your laboratory advisor
Precautions for Use
All biological samples must be considered as potentially infectious and handled with the usual precautions (compulsory wearing of gloves, hand washing, etc.).
Storage
- Store between 2°C and 8°C (R1 could be stored between 2 and 30°C)
- Keep away from light
- Do not use after the expiry date
- After opening, to be used within 1 month
- Shelf life: 12 months
- Guaranteed minimum remaining shelf life at delivery: 1/3 of the total shelf life
Waste management
After use, sterilize the cultures and all contaminated material.
Substance with hazardous characteristics. Please contact your watsan referent for advice on proper disposal.
Detailed hazard and precautionary information can be found in the safety data sheet (SDS).
Classification EC Regulation N° 1272/2008
R1:
- Skin corrosion/irritation, Category 1, H314
- Serious eye damage/ eye irritation, Category 1, H318
Signal Word
Danger
H314 | Causes severe skin burns and eye damage. |
H318 | Causes serious eye damage. |
P260 | Do not breathe dust/fume/gas/mist/vapours/spray. |
P264 | Wash … thoroughly after handling |
P280 | Wear protective gloves/protective clothing/eye protection/face protection |
P310 | Immediately call a POISON CENTER or doctor/physician. |
P363 | Wash contaminated clothing before reuse. |
P301 + P330 + P331 | IF SWALLOWED: rinse mouth. Do NOT induce vomiting |
P303 + P361 + P353 | IF ON SKIN (or hair): Remove/Take off immediately all contaminated clothing. |
P304 + P340 | IF INHALED: Remove to fresh air and keep at rest in a position comfortable for breathing. |
P305 + P351 + P338 | IF IN EYES: rinse cautiously with water for several minutes. If the victim wears contact lenses, remove them if possible. Keep rinsing |
P501 | Dispose of contents/container to location in accordance with local and regional/ national/ international regulations |
Signal Word
Warning
H315 | Causes skin irritation |
H319 | Causes serious eye irritation |
H335 | May cause respiratory irritation |
P260 | Do not breathe dust/fume/gas/mist/vapours/spray. |
P280 | Wear protective gloves/protective clothing/eye protection/face protection |
P302 + P352 | IF ON SKIN: wash with plenty of soap and water |
MSF requirements
Reserved for bacteriology programmes