AURAMINE M FLUORESCENT STAIN KIT, TB (BD), 3 x 250 ml 212519
Valid Article
FLUORESCENT STAIN KIT AURAMINE M (BD)
Definition
Fluorescent staining kit with auramine-rhodamine for the microscopic detection of mycobacteria.
Specifications
Technical specifications
Liquid ready to use reagents:
- TB Auramine M solution 1 x 250 ml
- Auramine O: 2g
- Phenol, USP: 4.0 g
- Glycerine, USP: 100.0 ml
- Isopropanol: 250.0 ml
- Distilled water: 650.0 ml
- TB Decolorizer TM 1 x 250 ml
- Hydrochloric Acid: 5.0 ml
- Isopropanol: 700.0 ml
- Distilled water: 300.0 ml
- TB Potassium permanganate (KMnO4) 1 x 250 ml
- Potassium permanganate: 5.0 g
- Distilled water: 1000.0 ml
Packaging & Labelling
3 bottles of 250 ml
Transport Dangerous Goods
- UN3316
- Class: 9
- Packing group: II
- Proper Shipping Name: Chemical kit
Instructions for use
To be used with a fluorescent microscope (see related articles below).
Kit sufficient for 60-65 preparations.
In routine laboratory after good quality staining is established, switch to home made auramine with auramine O and potassium permanganate which is much cheaper than using this ready to use kit.
Please consult the “Updated laboratory procedures, 2022” available online via the Laboratory working Group sharepoint page: Laboratory Procedures and Resources.
https://msfintl.sharepoint.com/sites/msfintlcommunities/LabWG/SitePages/Laboratory-Manual-page.aspx
For offline access, contact your laboratory advisor.
Storage
- Protected from humidity
- Keep container tightly closed
- Reagents that had been removed from the packing carton should be stored in the dark.
- Temperature between 15º and 30ºC
- Do not store together with oxidizing and acidic materials
- Shelf life: 12 months
- Guaranteed minimum remaining shelf life at delivery: 1/3 of total shelf life
Waste management
Substance with potenital carcinogenic properties (H350) and hazardous characteristics. Please contact your watsan referent for advice on proper disposal.
Detailed hazard and precautionary information can be found in the safety data sheet (SDS).
Classification EC Regulation N° 1272/2008
TB Auramine M
- Flammable liquid: Category 3
- Serieus eye damage/eye irritation: Category 2A
- Carcinogenicity: Category 2
- Specific target organ toxicity - single exposure: Category 3
TB Decolorizer TM
- Flammable liquid: Category 2
- Skin corrosion: Category 1
- Serious eye damage/Eye irritation: Category 1
- Specific target organ toxicity - single exposure: Category 3
TB Potassium permanganate
- Toxic to reproduction: Category 2
- Acute hazards to the aquatic environment: Category 2
- Chronic hazards to the aquatic environment: Category 3
Signal Word
TB Auramine M: Warning
H226 | Flammable liquid and vapour. |
H319 | Causes serious eye irritation |
H336 | May cause drowsiness or dizziness |
H351 | Suspected of causing cancer (state route of exposure if it is conclusively proven that no other routs of exposure cause the hazard). |
P403 | Store in a well-ventilated place. |
P405 | Store locked up. |
P201 | Obtain special instructions before use. |
P202 | Do not handle until all safety precautions have been read and understood. |
P210 | Keep away from heat/sparks/open flames/hot surfaces. – No smoking. |
P233 | Keep container tightly closed. |
P235 | Keep cool. |
P240 | Ground/bond container and receiving equipment. |
P241 | Use explosion-proof electrical/ventilating/lighting/.../ equipment. |
P242 | Use only non-sparking tools. |
P243 | Take precautionary measures against static discharge. |
P261 | Avoid breathing dust/fume/gas/mist/vapours/spray. |
P271 | Use only outdoors or in a well-ventilated area. |
P264 | Wash … thoroughly after handling |
P280 | Wear protective gloves/protective clothing/eye protection/face protection |
P312 | Call a POISON CENTER or doctor/physician if you feel unwell. |
P303 + P361 + P353 | IF ON SKIN (or hair): Remove/Take off immediately all contaminated clothing. |
P304 + P340 | IF INHALED: Remove to fresh air and keep at rest in a position comfortable for breathing. |
P305 + P351 + P338 | IF IN EYES: rinse cautiously with water for several minutes. If the victim wears contact lenses, remove them if possible. Keep rinsing |
P308 + P313 | IF exposed or concerned: Get medical advice/attention. |
P337 + P313 | If eye irritation persists: get medical advice/attention |
P370 + P378 | In case of fire: Use... for extinction. |
P501 | Dispose of contents/container to location in accordance with local and regional/ national/ international regulations |
Signal Word
TB Decolorizer solution TM: Danger
H225 | Highly flammable liquid and vapour |
H314 | Causes severe skin burns and eye damage. |
H336 | May cause drowsiness or dizziness |
P403 | Store in a well-ventilated place. |
P405 | Store locked up. |
P210 | Keep away from heat/sparks/open flames/hot surfaces. – No smoking. |
P233 | Keep container tightly closed. |
P235 | Keep cool. |
P240 | Ground/bond container and receiving equipment. |
P241 | Use explosion-proof electrical/ventilating/lighting/.../ equipment. |
P242 | Use only non-sparking tools. |
P243 | Take precautionary measures against static discharge. |
P260 | Do not breathe dust/fume/gas/mist/vapours/spray. |
P271 | Use only outdoors or in a well-ventilated area. |
P264 | Wash … thoroughly after handling |
P280 | Wear protective gloves/protective clothing/eye protection/face protection |
P310 | Immediately call a POISON CENTER or doctor/physician. |
P321 | Specific treatment (see ... on this label) |
P363 | Wash contaminated clothing before reuse. |
P301 + P330 + P331 | IF SWALLOWED: rinse mouth. Do NOT induce vomiting |
P303 + P361 + P353 | IF ON SKIN (or hair): Remove/Take off immediately all contaminated clothing. |
P304 + P340 | IF INHALED: Remove to fresh air and keep at rest in a position comfortable for breathing. |
P305 + P351 + P338 | IF IN EYES: rinse cautiously with water for several minutes. If the victim wears contact lenses, remove them if possible. Keep rinsing |
P370 + P378 | In case of fire: Use... for extinction. |
P501 | Dispose of contents/container to location in accordance with local and regional/ national/ international regulations |
Signal Word
TB Potassium permanganate: Warning
H361 | Suspected of damaging fertility or the unborn child (route of exposure if it is conclusively proven that no other routes of exposure cause the hazard). |
H412 | Harmful to aquatic life with long lasting effects |
H401 | Toxic to aquatic life |
P405 | Store locked up. |
P201 | Obtain special instructions before use. |
P202 | Do not handle until all safety precautions have been read and understood. |
P235 | Keep cool. |
P273 | Avoid release to the environment |
P280 | Wear protective gloves/protective clothing/eye protection/face protection |
P308 + P313 | IF exposed or concerned: Get medical advice/attention. |
P501 | Dispose of contents/container to location in accordance with local and regional/ national/ international regulations |
MSF requirements
Staining kit necessary for the detection of mycobacteria with a fluorescence microscope.