AURAMINE M FLUORESCENT STAIN KIT, TB (BD), 3 x 250 ml 212519

STD SLASAURA3K2

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CE marking: declaration that the product meets EU standards for health, safety, and environmental protection. The CE marking indicates that the product may be sold freely in any part of the European Economic Area, regardless of its country of origin.
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Shelf life = length of time a product can remain active and effective. Short shelf life = shelf life = < 24 months
W0105010799 - Mycobacteria reagents - other
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Hazardous materials and products (identified by their 4-digit transport UN-number) must be transported under certain conditions as specified in the MSDS sheet (Material Safety Data Sheet).
Thermosensitive codes are defined for storage and transportation temperature requirements of the products.
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FLUORESCENT STAIN KIT AURAMINE M (BD)

Definition

Fluorescent staining kit with auramine-rhodamine for the microscopic detection of mycobacteria.

Specifications

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Technical specifications

Liquid ready to use reagents:

  • TB Auramine M solution 1 x 250 ml
    • Auramine O: 2g
    • Phenol, USP: 4.0 g
    • Glycerine, USP: 100.0 ml
    • Isopropanol: 250.0 ml
    • Distilled water: 650.0 ml
  • TB Decolorizer TM 1 x 250 ml
    • Hydrochloric Acid: 5.0 ml
    • Isopropanol: 700.0 ml
    • Distilled water: 300.0 ml
  • TB Potassium permanganate (KMnO4) 1 x 250 ml
    • Potassium permanganate: 5.0 g
    • ​Distilled water: 1000.0 ml

Packaging & Labelling

3 bottles of 250 ml

Transport Dangerous Goods

  • UN3316
  • Class: 9
  • Packing group: II
  • Proper Shipping Name: Chemical kit

Instructions for use

To be used with a fluorescent microscope (see related articles below).

Kit sufficient for 60-65 preparations.

In routine laboratory after good quality staining is established, switch to home made auramine with auramine O and potassium permanganate which is much cheaper than using this ready to use kit.

Please consult the “Updated laboratory procedures, 2022” available online via the Laboratory working Group sharepoint page: Laboratory Procedures and Resources.

https://msfintl.sharepoint.com/sites/msfintlcommunities/LabWG/SitePages/Laboratory-Manual-page.aspx

For offline access, contact your laboratory advisor.

Storage

  • Protected from humidity
  • Keep container tightly closed
  • Reagents that had been removed from the packing carton should be stored in the dark.
  • Temperature between 15º and 30ºC
  • Do not store together with oxidizing and acidic materials
  • Shelf life: 12 months
  • Guaranteed minimum remaining shelf life at delivery: 1/3 of total shelf life

Waste management

Substance with potenital carcinogenic properties (H350) and hazardous characteristics. Please contact your watsan referent for advice on proper disposal.

Detailed hazard and precautionary information can be found in the safety data sheet (SDS).

Classification EC Regulation N° 1272/2008

TB Auramine M

  • Flammable liquid: Category 3
  • Serieus eye damage/eye irritation: Category 2A
  • Carcinogenicity: Category 2
  • Specific target organ toxicity - single exposure: Category 3

TB Decolorizer TM

  • Flammable liquid: Category 2
  • Skin corrosion: Category 1
  • Serious eye damage/Eye irritation: Category 1
  • Specific target organ toxicity - single exposure: Category 3

TB Potassium permanganate

  • Toxic to reproduction: Category 2
  • Acute hazards to the aquatic environment: Category 2
  • Chronic hazards to the aquatic environment: Category 3

Signal Word

TB Auramine M: Warning

Image Image Image
H226Flammable liquid and vapour.
H319Causes serious eye irritation
H336May cause drowsiness or dizziness
H351Suspected of causing cancer (state route of exposure if it is conclusively proven that no other routs of exposure cause the hazard).
P403Store in a well-ventilated place.
P405Store locked up.
P201Obtain special instructions before use.
P202Do not handle until all safety precautions have been read and understood.
P210Keep away from heat/sparks/open flames/hot surfaces. – No smoking.
P233Keep container tightly closed.
P235Keep cool.
P240Ground/bond container and receiving equipment.
P241Use explosion-proof electrical/ventilating/lighting/.../ equipment.
P242Use only non-sparking tools.
P243Take precautionary measures against static discharge.
P261Avoid breathing dust/fume/gas/mist/vapours/spray.
P271Use only outdoors or in a well-ventilated area.
P264Wash … thoroughly after handling
P280Wear protective gloves/protective clothing/eye protection/face protection
P312Call a POISON CENTER or doctor/physician if you feel unwell.
P303 + P361 + P353IF ON SKIN (or hair): Remove/Take off immediately all contaminated clothing.
P304 + P340IF INHALED: Remove to fresh air and keep at rest in a position comfortable for breathing.
P305 + P351 + P338IF IN EYES: rinse cautiously with water for several minutes. If the victim wears contact lenses, remove them if possible. Keep rinsing
P308 + P313IF exposed or concerned: Get medical advice/attention.
P337 + P313If eye irritation persists: get medical advice/attention
P370 + P378In case of fire: Use... for extinction.
P501Dispose of contents/container to location in accordance with local and regional/ national/ international regulations

Signal Word

TB Decolorizer solution TM: Danger

Image Image Image
H225Highly flammable liquid and vapour
H314Causes severe skin burns and eye damage.
H336May cause drowsiness or dizziness
P403Store in a well-ventilated place.
P405Store locked up.
P210Keep away from heat/sparks/open flames/hot surfaces. – No smoking.
P233Keep container tightly closed.
P235Keep cool.
P240Ground/bond container and receiving equipment.
P241Use explosion-proof electrical/ventilating/lighting/.../ equipment.
P242Use only non-sparking tools.
P243Take precautionary measures against static discharge.
P260Do not breathe dust/fume/gas/mist/vapours/spray.
P271Use only outdoors or in a well-ventilated area.
P264Wash … thoroughly after handling
P280Wear protective gloves/protective clothing/eye protection/face protection
P310Immediately call a POISON CENTER or doctor/physician.
P321Specific treatment (see ... on this label)
P363Wash contaminated clothing before reuse.
P301 + P330 + P331IF SWALLOWED: rinse mouth. Do NOT induce vomiting
P303 + P361 + P353IF ON SKIN (or hair): Remove/Take off immediately all contaminated clothing.
P304 + P340IF INHALED: Remove to fresh air and keep at rest in a position comfortable for breathing.
P305 + P351 + P338IF IN EYES: rinse cautiously with water for several minutes. If the victim wears contact lenses, remove them if possible. Keep rinsing
P370 + P378In case of fire: Use... for extinction.
P501Dispose of contents/container to location in accordance with local and regional/ national/ international regulations

Signal Word

TB Potassium permanganate: Warning

Image
H361Suspected of damaging fertility or the unborn child (route of exposure if it is conclusively proven that no other routes of exposure cause the hazard).
H412Harmful to aquatic life with long lasting effects
H401Toxic to aquatic life
P405Store locked up.
P201Obtain special instructions before use.
P202Do not handle until all safety precautions have been read and understood.
P235Keep cool.
P273Avoid release to the environment
P280Wear protective gloves/protective clothing/eye protection/face protection
P308 + P313IF exposed or concerned: Get medical advice/attention.
P501Dispose of contents/container to location in accordance with local and regional/ national/ international regulations

MSF requirements

Staining kit necessary for the detection of mycobacteria with a fluorescence microscope.

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