MUELLER HINTON II AGAR, dehydrated, 500g [BD-211438]


Valid Article

W0104010101 - Dehydrated culture media (dcm)
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Mueller Hinton media is used for standardised testing of antimicrobials susceptibility testing (AST) with diffusion method for non-fastidious microorganisms.

Mueller Hinton media complemented with horse blood/sheep blood is used for AST for fastidious microorganisms.


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Approximate formula after reconstitution (1 litre):

  • Beef extract 2.0 g
  • Acid hydrolysate of casein 17.5 g
  • Starch 1.5 g
  • Agar 17.0 g

Technical specifications

  • Dehydrated medium, powder
  • Fine, dry, homogeneous, free of extraneous material
  • Prepared appearance: Light to medium, yellow to tan, trace hazy to slightly hazy

Packaging & Labelling

Bottle of 500 g

Instructions for use

  • Follow the recommendations of the package insert.
  • Do not use non homogenous media
  • Properly close the bottle after use

Please consult the “Bacteriology laboratory procedures and resources” available online via the Laboratory working Group sharepoint page: Laboratory Procedures and Resources

For offline access, contact your laboratory advisor

Precautions for Use

All biological samples must be considered as potentially infectious and handled with the usual precautions (compulsory wearing of gloves, hand washing, etc.)


  • Follow the recommendations of the manufacturer for the storage
  • Preserved from humidity, store in cool and dry place
  • Keep conainer tightly closed
  • Do not use after expiry date
  • After preparation, store the media maximum 3 weeks between 2°C and 8°C

Waste management

After use, sterilize the cultures and all contaminated material. Please contact your watsan referent for advice on proper disposal.

MSF requirements

Reserved to bacteriology programmes

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