COLUMBIA CNA AGAR, dehydrated, 500g [BD-212104]
Valid Article
COLUMBIA CNA AGAR
Definition
Columbia CNA Agar (Columbia Colistin Nalidixic Acid Agar) is a selective and differential medium for gram-positive bacteria. It can be supplemented with 5% of sheep blood.
It contains two antibiotics, Colistine and Nalidixic Acid, which inhibit the growth of gram-negative bacteria, thus selecting for gram-positive organisms.
Specifications
Components
Approximate Formula Per Liter :
- Pancreatic digest of casein 12.0 g
- Peptic digest of animal tissue 5.0 g
- Yeast extract 3.0 g
- Beef extract 3.0 g
- Corn starch 1.0 g
- Sodium chloride. 5.0 g
- Agar 13.5 g
- Colistin 10.0 mg
- Nalidixic acid 10.0 mg
Technical specifications
- Powder: fine, homogeneous, free of extraneous material.
- Soluble in water.
- Prepared appearance: tan to yellow, hazy.
Packaging & Labelling
Bottle of 500g
Instructions for use
- Follow the recommendations of the package insert.
- Do not use non homogeneous media.
- Properly close the bottle after use
Please consult the “Bacteriology laboratory procedures and resources” available online via the Laboratory working Group sharepoint page: Laboratory Procedures and Resources
For offline access, contact your laboratory advisor
Precautions for Use
All biological samples must be considered as potentially infectious and handled with the usual precautions (compulsory wearing of gloves, hand washing, etc.).
Storage
- Follow the recommendations of the manufacturer for the storage.
- Do not freeze.
- Expiry date on the bottle.
- After preparation, store up to 3 weeks at 2°C to 8°C.
Waste management
After use, sterilize the cultures and all contaminated material. Please contact your watsan referent for advice on proper disposal.
MSF requirements
Reserved to bacteriology programmes.