COLUMBIA CNA AGAR, dehydrated, 500g [BD-212104]

STD SBCMAGARCNABD

Valid Article

W0104010101 - Dehydrated culture media (dcm)
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COLUMBIA CNA AGAR

Definition

Columbia CNA Agar (Columbia Colistin Nalidixic Acid Agar) is a selective and differential medium for gram-positive bacteria. It can be supplemented with 5% of sheep blood.

It contains two antibiotics, Colistine and Nalidixic Acid, which inhibit the growth of gram-negative bacteria, thus selecting for gram-positive organisms.

Specifications

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Components

Approximate Formula Per Liter :

  • Pancreatic digest of casein 12.0 g
  • Peptic digest of animal tissue 5.0 g
  • Yeast extract 3.0 g
  • Beef extract 3.0 g
  • Corn starch 1.0 g
  • Sodium chloride. 5.0 g
  • Agar 13.5 g
  • Colistin 10.0 mg
  • Nalidixic acid 10.0 mg

Technical specifications

  • Powder: fine, homogeneous, free of extraneous material.
  • Soluble in water.
  • Prepared appearance: tan to yellow, hazy.

Packaging & Labelling

Bottle of 500g

Instructions for use

  • Follow the recommendations of the package insert.
  • Do not use non homogeneous media.
  • Properly close the bottle after use

Please consult the “Bacteriology laboratory procedures and resources” available online via the Laboratory working Group sharepoint page: Laboratory Procedures and Resources

https://msfintl.sharepoint.com/sites/msfintlcommunities/LabWG/SitePages/Bacteriology-Laboratory-Resources.aspx

For offline access, contact your laboratory advisor

Precautions for Use

All biological samples must be considered as potentially infectious and handled with the usual precautions (compulsory wearing of gloves, hand washing, etc.).

Storage

  • Follow the recommendations of the manufacturer for the storage.
  • Do not freeze.
  • Expiry date on the bottle.
  • After preparation, store up to 3 weeks at 2°C to 8°C.

Waste management

After use, sterilize the cultures and all contaminated material. Please contact your watsan referent for advice on proper disposal.

MSF requirements

Reserved to bacteriology programmes.

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