COLUMBIA CNA AGAR, dehydrated, 500g [BD-212104]


Valid Article

W0104010101 - Dehydrated culture media (dcm)
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Columbia CNA Agar (Columbia Colistin Nalidixic Acid Agar) is a selective and differential medium for gram-positive bacteria. It can be supplemented with 5% of sheep blood.

It contains two antibiotics, Colistine and Nalidixic Acid, which inhibit the growth of gram-negative bacteria, thus selecting for gram-positive organisms.


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Approximate Formula Per Liter :

  • Pancreatic digest of casein 12.0 g
  • Peptic digest of animal tissue 5.0 g
  • Yeast extract 3.0 g
  • Beef extract 3.0 g
  • Corn starch 1.0 g
  • Sodium chloride. 5.0 g
  • Agar 13.5 g
  • Colistin 10.0 mg
  • Nalidixic acid 10.0 mg

Technical specifications

  • Powder: fine, homogeneous, free of extraneous material.
  • Soluble in water.
  • Prepared appearance: tan to yellow, hazy.

Packaging & Labelling

Bottle of 500g

Instructions for use

  • Follow the recommendations of the package insert.
  • Do not use non homogeneous media.
  • Properly close the bottle after use

Please consult the “Bacteriology laboratory procedures and resources” available online via the Laboratory working Group sharepoint page: Laboratory Procedures and Resources

For offline access, contact your laboratory advisor

Precautions for Use

All biological samples must be considered as potentially infectious and handled with the usual precautions (compulsory wearing of gloves, hand washing, etc.).


  • Follow the recommendations of the manufacturer for the storage.
  • Do not freeze.
  • Expiry date on the bottle.
  • After preparation, store up to 3 weeks at 2°C to 8°C.

Waste management

After use, sterilize the cultures and all contaminated material. Please contact your watsan referent for advice on proper disposal.

Skin sensitizer, category 1, H317


Signal Word


H317May cause an allergic skin reaction
P261Avoid breathing dust/fume/gas/mist/vapours/spray.
P272Contaminated work clothing should not be allowed out of the workplace.
P280Wear protective gloves/protective clothing/eye protection/face protection
P363Wash contaminated clothing before reuse.
P302 + P352IF ON SKIN: wash with plenty of soap and water
P333 + P313If skin irritation or rash occurs: Get medical advice/attention.
P501Dispose of contents/container to location in accordance with local and regional/ national/ international regulations

MSF requirements

Reserved to bacteriology programmes.

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