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HAT MODIFIED SIMPLE CENTRIFUGATION KIT, LCR

https://unicat.msf.org/cat/product/53994
STD ELABHATR1--

Valid Article

Former Code(s): ELAEHATR1-- ELAEZFR0404
Single use
A single-use device, also referred to as a disposable device, is intended for use on one patient during a single procedure. It is not intended to be reprocessed (i.e., cleaned and disinfected or sterilized).
P
The order of this product needs to be justified and is only acceptable under certain conditions.
OC subscriptions: included in supply or field order tools (e.g. UniField)
SC subscriptions: included in supply or field tools
OC validations: approved for procurement and use by an OC for international or local orders specifying context and activity in Medical Standard Lists (MSL)

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HAT Modified Simple Centrifugation CSF

Definition

The Modified Simple Centrifugation technique (MSC) is used for the detection of trypanosomes (Human African Trypanosomiasis‌ = HAT) in cerebro-spinal fluid (CSF).

The trypanosomes of the CSF are concentrated in the bottom of the collecting tube (same tube as the one used in the mAECT technique), by centrifugation. After centrifugation, the tip of the transparent tube is examined under the microscope for the presence of mobile trypanosomes.

The MSC technique is a simple and rapid alternative to the double centrifugation of CSF.

Specifications

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Components

One kit contains:

  • 20 collector tubes with blue stopper
  • 20 plastic graduated transfer pipettes
  • 20 centrifuge tubes
  • instruction leaflet

Technical specifications

  • Set to perform 20 tests on CSF sample (3.5 ml)
  • Collector tube
    • transparent plastic
    • blue stopper
    • non sterile, single use
  • Transfer pipette
    • transparent plastic
    • graduated
    • non sterile, single use
  • Centrifuge tube
    • round bottom
    • plastic
    • 14 ml
    • non sterile, single use

Packaging & Labelling

Kit including different components (see above)

To be Ordered Separately

  • HAT viewing / reading chamber
  • tube rack
  • microscope with 10x objective and 10x or 15x ocular
  • latex gloves
  • waste container
  • detergent-disinfectant solution
  • table centrifuge

Instructions for use

  • Follow the instructions of the package insert.
  • Open only the collector tubes just before use, to avoid any contamination.
  • Trypanosomes are fragile. CSF should be examined as soon as possible, no later than 30 minutes after collection.
  • Use sterile gloves for CSF sampling and test performance.

Storage

Store at room temperature

Waste management

Destroy waste material properly after use by incineration.

Non-disposable materials must be cleaned and disinfected with 0.5% detergent-disinfectant solution for medical equipment (i.e. 25 ml = 1 stroke of dosing pump for 5 litres water) and dried.

MSF requirements

Easy to use technique to concentrate the trypanosomes in CSF samples with high sensitivity.

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