LEISHMANIASIS Kala Azar TEST (DAT), freeze-dried, ser/wb,5ml
Valid Article
OC subscriptions: included in supply or field order tools (e.g. UniField)
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SC subscriptions: included in supply or field tools
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OC validations: approved for procurement and use by an OC for international or local orders specifying context and activity in Medical Standard Lists (MSL)
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LEISHMANIASIS Kala Azar TEST (DAT)
Definition
Freeze-dried solution containing the visceral leishmaniasis antigen (Kala Azar). Essential reagent for the DAT test (Direct Agglutination Test).
The antigen used is a trypsin treated promastigote obtained from Leishmania donovani, preserved in formalin, and stained with Coomassie blue (non infectious part).
The DAT is used as a second test, to retest the samples that test negative with IT LEISH RDT. It has a higher sensitivity so this test will detect false negatives.
Specifications
Technical specifications
- Sensitivity: 95%
- Specificity: 95-99%
- Freeze-dried antigen
- Parasite density: 5 x 107/ml
Packaging & Labelling
5 ml vial for 8 patients with 11 levels of dilution or for 12 patients with 8 levels of dilution.
Instructions for use
Follow the protocol provided with the test.
Can be performed on serum or whole blood.
Positive and negative controls have to be ordered separately (see related articles below).
Please consult the “Updated laboratory procedures, 2022” available online via the Laboratory working Group sharepoint page: Laboratory Procedures and Resources.
https://msfintl.sharepoint.com/sites/msfintlcommunities/LabWG/SitePages/Laboratory-Manual-page.aspx
For offline access, contact your laboratory advisor.
Precautions for Use
All biological samples must be considered as potentially infectious and handled with the usual precautions (compulsary wearing of gloves, hand washing, etc.).
Storage
- Store between 20 and 35ºC, in a dark place
- Shelf life: 24 months
- Once reconstituted, the solution should be kept refrigerated between 2º and 8ºC for 7 days max.
- Not to be used beyond expiry date
Waste management
Incinerate the tests and the used materials.
MSF requirements
Only antigen validated by the Institutes of Tropical Medicine (Antwerp and Amsterdam).
Freeze-dried antigen, stable and easy to use.
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