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HAT mAECT KIT, collector tube + mini-column + pipette + tube

https://unicat.msf.org/cat/product/53998
STD ELABHATR2--

Valid Article

Former Code(s): ELAEZFR0402 ELAEZFR0403 ELAEHATR2--
Single use
A single-use device, also referred to as a disposable device, is intended for use on one patient during a single procedure. It is not intended to be reprocessed (i.e., cleaned and disinfected or sterilized).
P
The order of this product needs to be justified and is only acceptable under certain conditions.
OC subscriptions: included in supply or field order tools (e.g. UniField)
SC subscriptions: included in supply or field tools
OC validations: approved for procurement and use by an OC for international or local orders specifying context and activity in Medical Standard Lists (MSL)

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HAT mAECT KIT, mini-column

Definition

The mini anion exchange centrifugation technique (mAECT) is used for the detection of trypanosomes (Human African Trypanosomiasis).

It consists of separating the trypanosomes from venous blood by anion exchange chromatography and concentrating them in the bottom of a transparent tube by centrifugation (3000 rpm). After centrifugation, the tip of the transparent tube is examined under the microscope for the presence of mobile trypanosomes.

Specifications

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Components

One kit contains:

  • 20 mini-column with 2 stoppers per column
  • 20 collector tubes with blue stopper
  • 20 plastic graduated transfer pipettes
  • 20 centrifuge tubes
  • instruction leaflet

Technical specifications

  • Set to perform 20 tests
  • High sensitivity: 500 µl of blood allows detection of less than 50 trypanosomes/ml
  • Mini-column (2)
    • plastic
    • single use, sterile
    • grey rubber upper stopper
    • white lower stopper
    • used to separate blood from tryponasomes by anion exchange chromatography
  • Collector tube (1)
    • transparent plastic
    • blue stopper
    • non sterile, single use
  • Transfer pipette (3)
    • transparent plastic
    • graduated
    • non sterile, single use
  • Centrifuge tube (also called 'waste tube') (4)
    • round bottom, 14 ml
    • plastic
    • non sterile

Packaging & Labelling

Kit including different components (see above)

Articles to be ordered separately:

  • mAECT viewing chamber
  • mAECT column/tube rack
  • mAECT column opener
  • microscope with 10x objective and 10x or 15x ocular
  • latex gloves
  • micropipette for 500 µl with pipette tips
  • waste container
  • detergent-disinfectant solution
  • electrical centrifuge

Instructions for use

  • Follow the instructions of the package insert.
  • Open only the minicolumns and collector tubes just before use, in order to avoid any contamination.
  • Trypanosomes are fragile. Blood should be examined as soon as possible, no later than 30 minutes after collection.
  • Use latex gloves for blood sampling and test performance.
  • Use the heparin lithium tubes for blood sampling. Do not use EDTA or citrate as anticoagulant.
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Maintenance

In case the device needs to be reused, it must be cleaned and disinfected with a 0.5% detergent-disinfectant solution for medical equipment (i.e. 25 ml = 1 stroke of dosing pump for 5 litres water).

(Cf Introduction: Disinfection and sterilization in the field)

Storage

  • Store mini-columns at 4°C
  • Do not freeze the mini-columns
  • Shelf life columns: 2 years

(Cf Introduction: Thermosensitive products)

Waste management

Destroy waste material properly after use by incineration.

MSF requirements

Easy to use technique to concentrate the trypanosomes in blood samples with high sensitivity. Despite logistical needs, it is necessary for sensitive diagnosis of HAT.

Can be performed in MSF trypanosomiasis programmes and is necessary for diagnosis of HAT with maximum sensitivity.

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